Ramond, JBLako, JDWStafford, William HLTuffin, MICowan, DA2015-10-152015-10-152015-02Ramond, JB, Lako, JDW, Stafford, WHL, Tuffin, MI and Cowan, DA. 2015. Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils. Journal of Basic Microbiology, Vol 55(8), pp 1040-10470233-111Xhttp://onlinelibrary.wiley.com/doi/10.1002/jobm.201400933/epdfhttp://hdl.handle.net/10204/8190Copyright: 2015. Wiley-VCH Verlag. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text I, tem. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in the Journal of Basic Microbiology, Vol 55(8), pp 1040-1047Ammonia-oxidizing bacteria (AOB) are essential in the biogeochemical cycling of nitrogen as they catalyze the rate-limiting oxidation of ammonia into nitrite. Since their first isolation in the late 19th century, chemolithoautotrophic AOBs have been identified in a wide range of natural (e.g. soils, sediments, estuarine, and freshwaters) and man created or impacted habitats (e.g. wastewater treatment plants and agricultural soils). However, little is known on the plant-species association of AOBs, particularly in the nutrient-starved fynbos terrestrial biome. In this study, we evaluated the diversity of AOBs in the plant canopy of three South African fynbos-specific plant species, namely Leucadendron xanthoconus, Leucospermum truncatulum and Leucadendron microcephalum, through the construction of amoA-gene clone libraries. Our results clearly demonstrate that plant-species specific and monophyletic AOB clades are present in fynbos canopy soils.enAmoA-gene diversityAmmonia oxidizersFynbos soilProteaceae familyPlant–microbe interactionsArticleRamond, J., Lako, J., Stafford, W. H., Tuffin, M., & Cowan, D. (2015). Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils. http://hdl.handle.net/10204/8190Ramond, JB, JDW Lako, William HL Stafford, MI Tuffin, and DA Cowan "Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils." (2015) http://hdl.handle.net/10204/8190Ramond J, Lako J, Stafford WH, Tuffin M, Cowan D. Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils. 2015; http://hdl.handle.net/10204/8190.TY - Article AU - Ramond, JB AU - Lako, JDW AU - Stafford, William HL AU - Tuffin, MI AU - Cowan, DA AB - Ammonia-oxidizing bacteria (AOB) are essential in the biogeochemical cycling of nitrogen as they catalyze the rate-limiting oxidation of ammonia into nitrite. Since their first isolation in the late 19th century, chemolithoautotrophic AOBs have been identified in a wide range of natural (e.g. soils, sediments, estuarine, and freshwaters) and man created or impacted habitats (e.g. wastewater treatment plants and agricultural soils). However, little is known on the plant-species association of AOBs, particularly in the nutrient-starved fynbos terrestrial biome. In this study, we evaluated the diversity of AOBs in the plant canopy of three South African fynbos-specific plant species, namely Leucadendron xanthoconus, Leucospermum truncatulum and Leucadendron microcephalum, through the construction of amoA-gene clone libraries. Our results clearly demonstrate that plant-species specific and monophyletic AOB clades are present in fynbos canopy soils. DA - 2015-02 DB - ResearchSpace DP - CSIR KW - AmoA-gene diversity KW - Ammonia oxidizers KW - Fynbos soil KW - Proteaceae family KW - Plant–microbe interactions LK - https://researchspace.csir.co.za PY - 2015 SM - 0233-111X T1 - Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils TI - Evidence of novel plant-species specific ammonia oxidizing bacterial clades in acidic South African fynbos soils UR - http://hdl.handle.net/10204/8190 ER -