Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation

Ashok, ACMarchington, RFMthunzi-Kufa, PatienceKrauss, TFDholakia, K2011-04-112011-04-112010-03Ashok, AC, Marchington, RF, Mthunzi, P et al. 2010. Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. Optics Express, Vol. 18(6), pp 6396-64071094-4087http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww%2Eopticsinfobase%2Eorg%2FDirectPDFAccess%2F3CD35F9C%2DCA1F%2DEF97%2DA8F24F0164B70D22%5F196601%2Epdf%3Fda%3D1%26id%3D196601%26seq%3D0%26mobile%3Dno&org=http://hdl.handle.net/10204/4952Copyright: 2010 Optical Society of America.The authors describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging.enOptical chromatographyOn chip fluorescence excitationPhotonic crystal fiber.Optical confinementOptical manipulationOptical tweezersOpticsOptical chromatography using a photonic crystal fiber with on-chip fluorescence excitationArticleAshok, A., Marchington, R., Mthunzi, P., Krauss, T., & Dholakia, K. (2010). Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. http://hdl.handle.net/10204/4952Ashok, AC, RF Marchington, P Mthunzi, TF Krauss, and K Dholakia "Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation." (2010) http://hdl.handle.net/10204/4952Ashok A, Marchington R, Mthunzi P, Krauss T, Dholakia K. Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation. 2010; http://hdl.handle.net/10204/4952.TY - Article AU - Ashok, AC AU - Marchington, RF AU - Mthunzi, P AU - Krauss, TF AU - Dholakia, K AB - The authors describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging. DA - 2010-03 DB - ResearchSpace DP - CSIR KW - Optical chromatography KW - On chip fluorescence excitation KW - Photonic crystal fiber. KW - Optical confinement KW - Optical manipulation KW - Optical tweezers KW - Optics LK - https://researchspace.csir.co.za PY - 2010 SM - 1094-4087 T1 - Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation TI - Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation UR - http://hdl.handle.net/10204/4952 ER -