Mthunzi-Kufa, PatienceForbes, AHawkins, DAbrahamse, HKarsten, AE2007-07-172007-07-172005Mthunzi, P, et al. 2005. Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts. Proceedings of SPIE, vol. 58760277-786Xhttp://hdl.handle.net/10204/1004Copyright: 2005 The International Society for Optical EngineeringA variety of strategies have been utilised for prevention and treatment of chronic wounds such as leg ulcers, diabetic foot ulcers and pressure sores1. Low Level Laser Therapy (LLLT) has been reported to be an invaluable tool in the enhancement of wound healing through stimulating cell proliferation, accelerating collagen synthesis and increasing ATP synthesis in mitochondria to name but a few2. This study focused on an in-vitro analysis of the cellular responses induced by treatment with three different laser beam profiles namely, the Gaussian (G), Super Gaussian (SG) and Truncated Gaussian (TG), on normal wounded irradiated (WI) and wounded non-irradiated (WNI) human skin fibroblast cells (WS1), to test their influence in wound healing at 632.8 nm using a helium neon (HeNe) laser. For each beam profile, measurements were made using average energy densities over the sample ranging from 0.2 to 1 J, with single exposures on normal wounded cells. The cells were subjected to different post irradiation incubation periods, ranging from 0 to 24 hours to evaluate the duration (time) dependent effects resulting from laser irradiation. The promoted cellular alterations were measured by increase in cell viability, cell proliferation and cytotoxicity. The results obtained showed that treatment with the G compared to the SG and TG beams resulted in a marked increase in cell viability and proliferation. The data also showed that when cells undergo laser irradiation some cellular processes are driven by the peak energy density rather than the energy of the laser beam. The authors show that there exist threshold values for damage, and suggest optimal operating regimes for laser based wound healing.enBeam shapeCell viabilityCell proliferationCytotoxicityLow level laser therapyFibroblastsIn-vitroConference PresentationMthunzi, P., Forbes, A., Hawkins, D., Abrahamse, H., & Karsten, A. (2005). Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts. The International Society for Optical Engineering. http://hdl.handle.net/10204/1004Mthunzi, P, A Forbes, D Hawkins, H Abrahamse, and AE Karsten. "Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts." (2005): http://hdl.handle.net/10204/1004Mthunzi P, Forbes A, Hawkins D, Abrahamse H, Karsten A, Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts; The International Society for Optical Engineering; 2005. http://hdl.handle.net/10204/1004 .TY - Conference Presentation AU - Mthunzi, P AU - Forbes, A AU - Hawkins, D AU - Abrahamse, H AU - Karsten, AE AB - A variety of strategies have been utilised for prevention and treatment of chronic wounds such as leg ulcers, diabetic foot ulcers and pressure sores1. Low Level Laser Therapy (LLLT) has been reported to be an invaluable tool in the enhancement of wound healing through stimulating cell proliferation, accelerating collagen synthesis and increasing ATP synthesis in mitochondria to name but a few2. This study focused on an in-vitro analysis of the cellular responses induced by treatment with three different laser beam profiles namely, the Gaussian (G), Super Gaussian (SG) and Truncated Gaussian (TG), on normal wounded irradiated (WI) and wounded non-irradiated (WNI) human skin fibroblast cells (WS1), to test their influence in wound healing at 632.8 nm using a helium neon (HeNe) laser. For each beam profile, measurements were made using average energy densities over the sample ranging from 0.2 to 1 J, with single exposures on normal wounded cells. The cells were subjected to different post irradiation incubation periods, ranging from 0 to 24 hours to evaluate the duration (time) dependent effects resulting from laser irradiation. The promoted cellular alterations were measured by increase in cell viability, cell proliferation and cytotoxicity. The results obtained showed that treatment with the G compared to the SG and TG beams resulted in a marked increase in cell viability and proliferation. The data also showed that when cells undergo laser irradiation some cellular processes are driven by the peak energy density rather than the energy of the laser beam. The authors show that there exist threshold values for damage, and suggest optimal operating regimes for laser based wound healing. DA - 2005 DB - ResearchSpace DP - CSIR KW - Beam shape KW - Cell viability KW - Cell proliferation KW - Cytotoxicity KW - Low level laser therapy KW - Fibroblasts KW - In-vitro LK - https://researchspace.csir.co.za PY - 2005 SM - 0277-786X T1 - Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts TI - Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts UR - http://hdl.handle.net/10204/1004 ER -