Oberholster, Paul JCheng, Po-HsunBotha, A-MGenthe, Bettina2014-07-222014-07-222014-09Oberholster, P.J., Cheng, P-H., Botha, A-M. and Genthe, B. 2013. The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions. Water Research, vol. 60, pp 82-920043-1354http://ac.els-cdn.com/S0043135414003169/1-s2.0-S0043135414003169-main.pdf?_tid=8b7169a6-10d4-11e4-b486-00000aab0f02&acdnat=1405946758_52d26d7a06be9deac07c52e78e287146http://hdl.handle.net/10204/7521Copyright: 2014 Elsevier. This is an ABSTRACT ONLY. The definitive version is published in Water Research, vol. 60, pp 82-92The metal bioaccumulation potential of selected macroalgae species at different pH ranges was study for usage as part of a possible secondary passive acid mine drainage (AMD) treatment technology in algae ponds. Two separate studies were conducted to determine the suitability of macroalgae for passive treatment when metabolic processes in macrophytes and microorganisms in constructed wetlands decrease during winter months. In the field study, the bioconcentration of metals (mg/kg dry weight) measured in the benthic macroalgae mats was in the following order: site Oedogonium crassum Al > Fe > Mn > Zn; site 2. Klebsormidium klebsii, Al > Fe > Mn > Zn; site 3. Microspora tumidula, Fe > Al > Mn > Zn and site 4. M. tumidula, Fe > Mn > Al > Zn. In the laboratory study, cultured macroalgae K. klebsii, O. crassum and M. tumidula isolated from the field sampling sites were exposed to three different pH values (3, 5 and 7), while bioaccumulation of the metals, Al, Fe, Mn and Zn and glutathione S-transferase (GST) activity were measured in the different selected algae species at a constant water temperature of 14 °C. Bioaccumulation of Al was the highest for O. crassum followed by K. klebsii and M. tumidula (p < 0.0001). From the study it was evident that the highest metal bioaccumulation occurred in the macroalgae O. crassum at all three tested pH values under constant low water temperature.enAcidophilic filamentous macroalgaeAcid Mine DrainageAMDBioaccumulationOxidative stressThe potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regionsArticleOberholster, P. J., Cheng, P., Botha, A., & Genthe, B. (2014). The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions. http://hdl.handle.net/10204/7521Oberholster, Paul J, Po-Hsun Cheng, A-M Botha, and Bettina Genthe "The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions." (2014) http://hdl.handle.net/10204/7521Oberholster PJ, Cheng P, Botha A, Genthe B. The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions. 2014; http://hdl.handle.net/10204/7521.TY - Article AU - Oberholster, Paul J AU - Cheng, Po-Hsun AU - Botha, A-M AU - Genthe, Bettina AB - The metal bioaccumulation potential of selected macroalgae species at different pH ranges was study for usage as part of a possible secondary passive acid mine drainage (AMD) treatment technology in algae ponds. Two separate studies were conducted to determine the suitability of macroalgae for passive treatment when metabolic processes in macrophytes and microorganisms in constructed wetlands decrease during winter months. In the field study, the bioconcentration of metals (mg/kg dry weight) measured in the benthic macroalgae mats was in the following order: site Oedogonium crassum Al > Fe > Mn > Zn; site 2. Klebsormidium klebsii, Al > Fe > Mn > Zn; site 3. Microspora tumidula, Fe > Al > Mn > Zn and site 4. M. tumidula, Fe > Mn > Al > Zn. In the laboratory study, cultured macroalgae K. klebsii, O. crassum and M. tumidula isolated from the field sampling sites were exposed to three different pH values (3, 5 and 7), while bioaccumulation of the metals, Al, Fe, Mn and Zn and glutathione S-transferase (GST) activity were measured in the different selected algae species at a constant water temperature of 14 °C. Bioaccumulation of Al was the highest for O. crassum followed by K. klebsii and M. tumidula (p < 0.0001). From the study it was evident that the highest metal bioaccumulation occurred in the macroalgae O. crassum at all three tested pH values under constant low water temperature. DA - 2014-09 DB - ResearchSpace DP - CSIR KW - Acidophilic filamentous macroalgae KW - Acid Mine Drainage KW - AMD KW - Bioaccumulation KW - Oxidative stress LK - https://researchspace.csir.co.za PY - 2014 SM - 0043-1354 T1 - The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions TI - The potential of selected macroalgal species for treatment of AMD at different pH ranges in temperate regions UR - http://hdl.handle.net/10204/7521 ER -