Van der Westhuizen, MSteenkamp, Lucia HSteenkamp, PApostolides, Z2016-07-222016-07-222016-03Van der Westhuizen, M. Steenkamp, L.H. Steenkamp, P. and Apostolides, Z. 2016. Alternative pathway implicated as an influencing factor in the synthesis of theaflavin. Biocatalysis and Biotransformation, 33(5-6), 298-3091024-2422http://www.tandfonline.com/doi/abs/10.3109/10242422.2016.1163341?journalCode=ibab20http://hdl.handle.net/10204/8686Copyright: 2016 Taylor & Francis. . Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Biocatalysis and Biotransformation,33(5-6), 298-309The principal pigments present in black tea, theaflavins (TF), have been indicated to be of potential clinical significance in various fields of research which has been hampered by the very low levels of TFs from black tea extractions, being the original method employed to acquire TFs. Forelle pear (44µM TF/g dry weight/h) and Yacon leaf (65µM TF/g dry weight/h) homogenates were tested for their TF synthesis capacity and found to have a larger TF synthesis capacity than a green tea leaf homogenate (26µM TF/g dry weight/h) based upon the flavognost method. In an incubation system of green tea leaf extract utilizing endogenous enzymes present in Forelle pear and Yacon homogenates to synthesize TF, the formation of an unknown peak [m/z 563.1349; (23.95)(sup5); C(sub26)H(sub28)O(sub14)] was detected by mass spectrometry with a molecular mass similar to TF. This is in contrast to TF being solely synthesized in an in vitro model incubation system using isolated catechins and purified Forelle pear polyphenol oxidase. The preferential formation of the unknown compound could explain the low levels of TFs in black tea.enBioconversionForelle pearPolyphenol oxidaseTheaflavinsYaconArticleVan der Westhuizen, M., Steenkamp, L. H., Steenkamp, P., & Apostolides, Z. (2016). Alternative pathway implicated as an influencing factor in the synthesis of theaflavin. http://hdl.handle.net/10204/8686Van der Westhuizen, M, Lucia H Steenkamp, P Steenkamp, and Z Apostolides "Alternative pathway implicated as an influencing factor in the synthesis of theaflavin." (2016) http://hdl.handle.net/10204/8686Van der Westhuizen M, Steenkamp LH, Steenkamp P, Apostolides Z. Alternative pathway implicated as an influencing factor in the synthesis of theaflavin. 2016; http://hdl.handle.net/10204/8686.TY - Article AU - Van der Westhuizen, M AU - Steenkamp, Lucia H AU - Steenkamp, P AU - Apostolides, Z AB - The principal pigments present in black tea, theaflavins (TF), have been indicated to be of potential clinical significance in various fields of research which has been hampered by the very low levels of TFs from black tea extractions, being the original method employed to acquire TFs. Forelle pear (44µM TF/g dry weight/h) and Yacon leaf (65µM TF/g dry weight/h) homogenates were tested for their TF synthesis capacity and found to have a larger TF synthesis capacity than a green tea leaf homogenate (26µM TF/g dry weight/h) based upon the flavognost method. In an incubation system of green tea leaf extract utilizing endogenous enzymes present in Forelle pear and Yacon homogenates to synthesize TF, the formation of an unknown peak [m/z 563.1349; (23.95)(sup5); C(sub26)H(sub28)O(sub14)] was detected by mass spectrometry with a molecular mass similar to TF. This is in contrast to TF being solely synthesized in an in vitro model incubation system using isolated catechins and purified Forelle pear polyphenol oxidase. The preferential formation of the unknown compound could explain the low levels of TFs in black tea. DA - 2016-03 DB - ResearchSpace DP - CSIR KW - Bioconversion KW - Forelle pear KW - Polyphenol oxidase KW - Theaflavins KW - Yacon LK - https://researchspace.csir.co.za PY - 2016 SM - 1024-2422 T1 - Alternative pathway implicated as an influencing factor in the synthesis of theaflavin TI - Alternative pathway implicated as an influencing factor in the synthesis of theaflavin UR - http://hdl.handle.net/10204/8686 ER -