Wheeler, JWong, AMarondedze, CGroen, AJKwezi, Lusisizwe2017-08-222017-08-222017-06Wheeler, J., Wong, A., Marondedze, C. et al. 2017. The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling. The Plant Journal, vol. 91 (4): 590-600. DOI: 10.1111/tpj.135890960-7412DOI: 10.1111/tpj.13589http://onlinelibrary.wiley.com/doi/10.1111/tpj.13589/abstracthttp://hdl.handle.net/10204/9493Copyright: 2017 The AuthorsThe brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine-rich repeat receptor-like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per µg protein with a preference for magnesium over manganese as a co-factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI1 is modulated by the kinase while cGMP, the product of the guanylate cyclase, in turn inhibits BRI1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor.enArabidopsis thalianacGMPBrassinosteroid receptorBRI1Brassinosteroid signaling kinase 1BSK1Guanylate cyclaseKinaseArticleWheeler, J., Wong, A., Marondedze, C., Groen, A., & Kwezi, L. (2017). The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling. http://hdl.handle.net/10204/9493Wheeler, J, A Wong, C Marondedze, AJ Groen, and Lusisizwe Kwezi "The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling." (2017) http://hdl.handle.net/10204/9493Wheeler J, Wong A, Marondedze C, Groen A, Kwezi L. The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling. 2017; http://hdl.handle.net/10204/9493.TY - Article AU - Wheeler, J AU - Wong, A AU - Marondedze, C AU - Groen, AJ AU - Kwezi, Lusisizwe AB - The brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine-rich repeat receptor-like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per µg protein with a preference for magnesium over manganese as a co-factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI1 is modulated by the kinase while cGMP, the product of the guanylate cyclase, in turn inhibits BRI1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor. DA - 2017-06 DB - ResearchSpace DP - CSIR KW - Arabidopsis thaliana KW - cGMP KW - Brassinosteroid receptor KW - BRI1 KW - Brassinosteroid signaling kinase 1 KW - BSK1 KW - Guanylate cyclase KW - Kinase LK - https://researchspace.csir.co.za PY - 2017 SM - 0960-7412 T1 - The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling TI - The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling UR - http://hdl.handle.net/10204/9493 ER -