Gbashi, SNjobeh, PSteenkamp, Paul AMadala, N2018-07-092018-07-092017Gbashi, S. et al. 2017. Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry. CyTA - Journal of Food, vol. 15(2): 171-1801947-63371947-6345doi.org/10.1080/19476337.2016.1230151http://www.tandfonline.com/doi/full/10.1080/19476337.2016.1230151http://hdl.handle.net/10204/10293© 2016 The Author(s).The need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C and 150°C. The extracts were analyzed on UPLC-qTOF-MS/MS and 28 flavonoids of different classes were identified. Further analysis of the data using principal component analysis revealed differential distribution patterns of the identified molecules. In overall, the extraction yield increased proportionately with increasing temperature. It can thus be deduced that PHWE is an excellent extraction method of flavonoids from plant tissues. Again, this study reiterates B. pilosa as a rich source of flavonoids.enGreen extraction proceduresBidens pilosaFlavonoidsPressurized hot waterArticleGbashi, S., Njobeh, P., Steenkamp, P. A., & Madala, N. (2017). Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry. http://hdl.handle.net/10204/10293Gbashi, S, P Njobeh, Paul A Steenkamp, and N Madala "Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry." (2017) http://hdl.handle.net/10204/10293Gbashi S, Njobeh P, Steenkamp PA, Madala N. Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry. 2017; http://hdl.handle.net/10204/10293.TY - Article AU - Gbashi, S AU - Njobeh, P AU - Steenkamp, Paul A AU - Madala, N AB - The need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C and 150°C. The extracts were analyzed on UPLC-qTOF-MS/MS and 28 flavonoids of different classes were identified. Further analysis of the data using principal component analysis revealed differential distribution patterns of the identified molecules. In overall, the extraction yield increased proportionately with increasing temperature. It can thus be deduced that PHWE is an excellent extraction method of flavonoids from plant tissues. Again, this study reiterates B. pilosa as a rich source of flavonoids. DA - 2017 DB - ResearchSpace DP - CSIR KW - Green extraction procedures KW - Bidens pilosa KW - Flavonoids KW - Pressurized hot water LK - https://researchspace.csir.co.za PY - 2017 SM - 1947-6337 SM - 1947-6345 T1 - Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry TI - Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry UR - http://hdl.handle.net/10204/10293 ER -