Sari-Ak, DAlomari, OShomali, RALim, JThimiri Govindaraj, Deepak B2023-03-172023-03-172022-12Sari-Ak, D., Alomari, O., Shomali, R., Lim, J. & Thimiri Govindaraj, D.B. 2022. Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review. <i>Viruses-Basel.</i> http://hdl.handle.net/10204/126791999-4915https://doi.org/10.3390/v15010054http://hdl.handle.net/10204/12679The baculovirus expression vector systems (BEVS) have been widely used for the recombinant production of proteins in insect cells and with high insert capacity. However, baculovirus does not replicate in mammalian cells; thus, the BacMam system, a heterogenous expression system that can infect certain mammalian cells, was developed. Since then, the BacMam system has enabled transgene expression via mammalian-specific promoters in human cells, and later, the MultiBacMam system enabled multi-protein expression in mammalian cells. In this review, we will cover the continual development of the BEVS in combination with CRPISPR-Cas technologies to drive genome-editing in mammalian cells. Additionally, we highlight the use of CRISPR-Cas in glycoengineering to potentially produce a new class of glycoprotein medicines in insect cells. Moreover, we anticipate CRISPR-Cas9 to play a crucial role in the development of protein expression systems, gene therapy, and advancing genome engineering applications in the future.FulltextenBaculovirus Vector SystemBEVSCRISPR-Cas9Complex GlycosylationMultiBacArticleSari-Ak, D., Alomari, O., Shomali, R., Lim, J., & Thimiri Govindaraj, D. B. (2022). Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review. <i>Viruses-Basel</i>, http://hdl.handle.net/10204/12679Sari-Ak, D, O Alomari, RA Shomali, J Lim, and Deepak B Thimiri Govindaraj "Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review." <i>Viruses-Basel</i> (2022) http://hdl.handle.net/10204/12679Sari-Ak D, Alomari O, Shomali R, Lim J, Thimiri Govindaraj DB. Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review. Viruses-Basel. 2022; http://hdl.handle.net/10204/12679.TY - Article AU - Sari-Ak, D AU - Alomari, O AU - Shomali, RA AU - Lim, J AU - Thimiri Govindaraj, Deepak B AB - The baculovirus expression vector systems (BEVS) have been widely used for the recombinant production of proteins in insect cells and with high insert capacity. However, baculovirus does not replicate in mammalian cells; thus, the BacMam system, a heterogenous expression system that can infect certain mammalian cells, was developed. Since then, the BacMam system has enabled transgene expression via mammalian-specific promoters in human cells, and later, the MultiBacMam system enabled multi-protein expression in mammalian cells. In this review, we will cover the continual development of the BEVS in combination with CRPISPR-Cas technologies to drive genome-editing in mammalian cells. Additionally, we highlight the use of CRISPR-Cas in glycoengineering to potentially produce a new class of glycoprotein medicines in insect cells. Moreover, we anticipate CRISPR-Cas9 to play a crucial role in the development of protein expression systems, gene therapy, and advancing genome engineering applications in the future. DA - 2022-12 DB - ResearchSpace DP - CSIR J1 - Viruses-Basel KW - Baculovirus Vector System KW - BEVS KW - CRISPR-Cas9 KW - Complex Glycosylation KW - MultiBac LK - https://researchspace.csir.co.za PY - 2022 SM - 1999-4915 T1 - Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review TI - Advances in CRISPR-Cas9 for the Baculovirus Vector System: A systematic review UR - http://hdl.handle.net/10204/12679 ER -26474