Wiederoder, MSSmith, SuzanneMadzivhandila, PhophiMager, DMoodley, KavandrenDeVoe, DLLand, Kevin J2018-09-182018-09-182017-09Wiederoder, M.S. et al. 2017. Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement. Biomicrofluidics, vol. 11(5): https://doi.org/10.1063/1.50026441932-1058https://doi.org/10.1063/1.5002644https://aip.scitation.org/doi/10.1063/1.5002644http://hdl.handle.net/10204/10410Copyright: 2017 The Authors.The presented work demonstrates novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement that leverage the advantages of both paper-based and centrifugal microfluidic platforms. The fluid flow is manipulated by balancing the capillary force of paper inserts with the centrifugal force generated by disc rotation to enhance the signal of a colorimetric lateral flow immunoassay for pathogenic E. coli. Low-cost centrifugation for pre-concentration of bacteria was demonstrated by sample sedimentation at high rotational speeds before supernatant removal by a paper insert via capillary force after deceleration. The live bacteria capture efficiency of the device was similar to a commercial centrifuge. This pre-concentrated sample when combined with gold nanoparticle immunoconjugate probes resulted in a detection limit that is 10 lower than a non-concentrated sample for a lateral flow immunoassay. Signal enhancement was also demonstrated through rotational speed variation to prevent the flow for on-device incubation and to reduce the flow rate, thus increasing the sample residence time for the improved capture of gold nanoparticle-bacteria complexes in an integrated paper microfluidic assay. Finally, multiple sequential steps including sample pre-concentration, filtration, incubation, target capture by an integrated paper microfluidic assay, silver enhancement and quenching, and index matching were completed within a single device. The detection limit was 105 colony forming units per ml, a 100 improvement over a similar paper-based lateral flow assay. The techniques utilize the advantages of paper-based microfluidic devices, while facilitating additional functionalities with a centrifugal microfluidic platform for detection performance enhancement in a low-cost, automated platform amenable to point-of-care environments.esHybrid paper-polymer centrifugal devicesPoint-of-care environmentsArticleWiederoder, M., Smith, S., Madzivhandila, P., Mager, D., Moodley, K., DeVoe, D., & Land, K. J. (2017). Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement. http://hdl.handle.net/10204/10410Wiederoder, MS, Suzanne Smith, Phophi Madzivhandila, D Mager, Kavandren Moodley, DL DeVoe, and Kevin J Land "Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement." (2017) http://hdl.handle.net/10204/10410Wiederoder M, Smith S, Madzivhandila P, Mager D, Moodley K, DeVoe D, et al. Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement. 2017; http://hdl.handle.net/10204/10410.TY - Article AU - Wiederoder, MS AU - Smith, Suzanne AU - Madzivhandila, Phophi AU - Mager, D AU - Moodley, Kavandren AU - DeVoe, DL AU - Land, Kevin J AB - The presented work demonstrates novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement that leverage the advantages of both paper-based and centrifugal microfluidic platforms. The fluid flow is manipulated by balancing the capillary force of paper inserts with the centrifugal force generated by disc rotation to enhance the signal of a colorimetric lateral flow immunoassay for pathogenic E. coli. Low-cost centrifugation for pre-concentration of bacteria was demonstrated by sample sedimentation at high rotational speeds before supernatant removal by a paper insert via capillary force after deceleration. The live bacteria capture efficiency of the device was similar to a commercial centrifuge. This pre-concentrated sample when combined with gold nanoparticle immunoconjugate probes resulted in a detection limit that is 10 lower than a non-concentrated sample for a lateral flow immunoassay. Signal enhancement was also demonstrated through rotational speed variation to prevent the flow for on-device incubation and to reduce the flow rate, thus increasing the sample residence time for the improved capture of gold nanoparticle-bacteria complexes in an integrated paper microfluidic assay. Finally, multiple sequential steps including sample pre-concentration, filtration, incubation, target capture by an integrated paper microfluidic assay, silver enhancement and quenching, and index matching were completed within a single device. The detection limit was 105 colony forming units per ml, a 100 improvement over a similar paper-based lateral flow assay. The techniques utilize the advantages of paper-based microfluidic devices, while facilitating additional functionalities with a centrifugal microfluidic platform for detection performance enhancement in a low-cost, automated platform amenable to point-of-care environments. DA - 2017-09 DB - ResearchSpace DP - CSIR KW - Hybrid paper-polymer centrifugal devices KW - Point-of-care environments LK - https://researchspace.csir.co.za PY - 2017 SM - 1932-1058 T1 - Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement TI - Novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement UR - http://hdl.handle.net/10204/10410 ER -