A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis

Ngamsom, BTruyts, Alma EFourie, Louis JKumar, ShavonIles, AMoodley, KlariskaLand, Kevin JPamme, NTarn, MD2017-11-222017-11-222017-08Ngamsom, B. et al. 2017. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry - A European Journal, vol. 23: DOI: 10.1002/chem.2017034870947-6539http://onlinelibrary.wiley.com/doi/10.1002/chem.201703487/fullDOI: 10.1002/chem.201703487http://hdl.handle.net/10204/9814Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(sup4) CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.enMicrofluidic systemsEscherichia coliImmunomagnetic separationIMSA microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysisArticleNgamsom, B., Truyts, A. E., Fourie, L. J., Kumar, S., Iles, A., Moodley, K., ... Tarn, M. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/9814Ngamsom, B, Alma E Truyts, Louis J Fourie, Shavon Kumar, A Iles, Klariska Moodley, Kevin J Land, N Pamme, and MD Tarn "A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis." (2017) http://hdl.handle.net/10204/9814Ngamsom B, Truyts AE, Fourie LJ, Kumar S, Iles A, Moodley K, et al. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. 2017; http://hdl.handle.net/10204/9814.TY - Article AU - Ngamsom, B AU - Truyts, Alma E AU - Fourie, Louis J AU - Kumar, Shavon AU - Iles, A AU - Moodley, Klariska AU - Land, Kevin J AU - Pamme, N AU - Tarn, MD AB - We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(sup4) CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring. DA - 2017-08 DB - ResearchSpace DP - CSIR KW - Microfluidic systems KW - Escherichia coli KW - Immunomagnetic separation KW - IMS LK - https://researchspace.csir.co.za PY - 2017 SM - 0947-6539 T1 - A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis TI - A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis UR - http://hdl.handle.net/10204/9814 ER -