Berger, EDu Plessis, EGerber, ICrampton, Michael CNxumalo, NPLouw, M2009-04-142009-04-142009-01Berger, E., Du Plessis, E., Gerber, I., et al. 2009. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. Applied and Environmental Microbiology, Vol. 75(1), pp 1-230099-2240http://hdl.handle.net/10204/3314Copyright: 2009 American Society for MicrobiologyThe flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteasesenBacillus haloduransGene encodingExtracellular proteasesFlagellin-fusionModified flagellinPeptide expressionMicrobiologyArticleBerger, E., Du Plessis, E., Gerber, I., Crampton, M. C., Nxumalo, N., & Louw, M. (2009). Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. http://hdl.handle.net/10204/3314Berger, E, E Du Plessis, I Gerber, Michael C Crampton, NP Nxumalo, and M Louw "Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression." (2009) http://hdl.handle.net/10204/3314Berger E, Du Plessis E, Gerber I, Crampton MC, Nxumalo N, Louw M. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. 2009; http://hdl.handle.net/10204/3314.TY - Article AU - Berger, E AU - Du Plessis, E AU - Gerber, I AU - Crampton, Michael C AU - Nxumalo, NP AU - Louw, M AB - The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases DA - 2009-01 DB - ResearchSpace DP - CSIR KW - Bacillus halodurans KW - Gene encoding KW - Extracellular proteases KW - Flagellin-fusion KW - Modified flagellin KW - Peptide expression KW - Microbiology LK - https://researchspace.csir.co.za PY - 2009 SM - 0099-2240 T1 - Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression TI - Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression UR - http://hdl.handle.net/10204/3314 ER -