Mthunzi-Kufa, Patience2016-06-272016-06-272012-08Mthunzi, P. 2012. The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation. In: Proceedings of SPIE 8458. Optical Trapping and Optical Micromanipulation IX, 12 August 2012, San Diego, California, USA.http://spie.org/Publications/Proceedings/Paper/10.1117/12.929673http://hdl.handle.net/10204/8556Proceedings of SPIE 8458. Optical Trapping and Optical Micromanipulation IX, 12 August 2012, San Diego, California, USA. The attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website.Stem cells are rich in proteins, carbohydrates, deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and various other cellular components which are responsible for a diversity of functions. Mostly the building blocks of these intracellular entities play an active role in absorbing ultra-violet (UV) and visible light sources. Light-matter interactions in biomaterials are a complex situation and subsequent damage may not always amount only from wavelength dependent effects but may also be driven by a wealth of other optical parameters which may lead to a variety photochemical reactions. Previously, literature has reported efficient photo-transfection and differentiation of pluripotent stem cells via near infrared (NIR) femtosecond (fs) laser pulses with minimum compromise to their viability. Therefore, in this study the influence of using different fs laser wavelengths on optical stem cell transfection and differentiation is investigated. A potassium titanyl phosphate (KTP) crystal was employed in frequency doubling a 1064 nm fs laser beam. The newly generated 532 nm fs pulsed beam was then utilized for the first time in transient photo-transfection of ES-E14TG2a mouse embryonic stem (mES) cells. Compared to using 1064 nm fs pulses which non-invasively introduce plasmid DNA and other macromolecules into mES cells, our results showed a significant decline in the photo-transfection efficiency following transfecting with a pulsed fs visible green beam.enEfficient photo-transfectionFrequency doublingKTP crystalLaser wavelength1064 nm laser beam532 nm laser beamBiomaterialsEmbryonic stem cellsPluripotent cellsLight-matter interactionotochemical reactionsConference PresentationMthunzi, P. (2012). The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation. http://hdl.handle.net/10204/8556Mthunzi, P. "The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation." (2012): http://hdl.handle.net/10204/8556Mthunzi P, The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation; 2012. http://hdl.handle.net/10204/8556 .TY - Conference Presentation AU - Mthunzi, P AB - Stem cells are rich in proteins, carbohydrates, deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and various other cellular components which are responsible for a diversity of functions. Mostly the building blocks of these intracellular entities play an active role in absorbing ultra-violet (UV) and visible light sources. Light-matter interactions in biomaterials are a complex situation and subsequent damage may not always amount only from wavelength dependent effects but may also be driven by a wealth of other optical parameters which may lead to a variety photochemical reactions. Previously, literature has reported efficient photo-transfection and differentiation of pluripotent stem cells via near infrared (NIR) femtosecond (fs) laser pulses with minimum compromise to their viability. Therefore, in this study the influence of using different fs laser wavelengths on optical stem cell transfection and differentiation is investigated. A potassium titanyl phosphate (KTP) crystal was employed in frequency doubling a 1064 nm fs laser beam. The newly generated 532 nm fs pulsed beam was then utilized for the first time in transient photo-transfection of ES-E14TG2a mouse embryonic stem (mES) cells. Compared to using 1064 nm fs pulses which non-invasively introduce plasmid DNA and other macromolecules into mES cells, our results showed a significant decline in the photo-transfection efficiency following transfecting with a pulsed fs visible green beam. DA - 2012-08 DB - ResearchSpace DP - CSIR KW - Efficient photo-transfection KW - Frequency doubling KW - KTP crystal KW - Laser wavelength KW - 1064 nm laser beam KW - 532 nm laser beam KW - Biomaterials KW - Embryonic stem cells KW - Pluripotent cells KW - Light-matter interaction KW - otochemical reactions LK - https://researchspace.csir.co.za PY - 2012 T1 - The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation TI - The influence of femtosecond laser pulse wavelength on embryonic stem cell differentiation UR - http://hdl.handle.net/10204/8556 ER -