Alexandre, Kabamba BGray, ESPantophlet, RMoore, PLMcMahon, JBChakauya, EO'Keefe, BRChikwamba, Rachel KMorris, L2013-05-272013-05-272011-09Alexandre, K.B, Gray, E.S, Pantophlet, R, Moore, P.L, McMahon, J.B, Chakauya, E, O'Keefe, B.R, Chikwamba, R and Morris, L. 2011. Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site. Journal of Virology, vol. 85(17), pp 9039-90500022-538Xhttp://jvi.asm.org/content/85/17/9039.abstracthttp://hdl.handle.net/10204/6755Copyright: 2011. American Society for Microbiology. This is the Pre/Post print version of the work. The definitive version is published in Journal of Virology, vol. 85(17), pp 9039-9050The glycans on HIV-1 gp120 play an important role in shielding neutralization sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study we investigated the interaction of the lectin griffithsin (GRFT) with HIV-1 gp120 and its effects on exposure of the CD4-binding site (CD4bs). We found that GRFT enhanced the binding of HIV-1 onto plates coated with anti-CD4bs antibodies b12, b6 or the CD4 receptor mimetic, CD4-IgG2. The average enhancement of b12 or b6 binding was higher for subtype B viruses compared to subtype C while for CD4-IgG2 it was similar for both subtypes, although lower than observed with antibodies. This GRFT-mediated enhancement of HIV-1 binding to b12 was reflected in synergistic neutralization for 2 of the 4 viruses tested. The glycan at position 386 which shields the CD4bs was involved in both GRFT mediated enhancement of binding as well as neutralization synergism between GRFT and b12. Although GRFT enhanced CD4bs exposure, it simultaneously inhibited ligand binding to the coreceptor binding site, suggesting that GRFT-dependent enhancement and neutralization utilize independent mechanisms. This study shows for the first time that GRFT interaction with gp120 exposes the CD4bs through binding the glycan at position 386 which may have implications for how to access this conserved site.enGriffithsinHIV-1 subtype CIgG1b12CD4 binding siteGlycansArticleAlexandre, K. B., Gray, E., Pantophlet, R., Moore, P., McMahon, J., Chakauya, E., ... Morris, L. (2011). Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site. http://hdl.handle.net/10204/6755Alexandre, Kabamba B, ES Gray, R Pantophlet, PL Moore, JB McMahon, E Chakauya, BR O'Keefe, Rachel K Chikwamba, and L Morris "Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site." (2011) http://hdl.handle.net/10204/6755Alexandre KB, Gray E, Pantophlet R, Moore P, McMahon J, Chakauya E, et al. Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site. 2011; http://hdl.handle.net/10204/6755.TY - Article AU - Alexandre, Kabamba B AU - Gray, ES AU - Pantophlet, R AU - Moore, PL AU - McMahon, JB AU - Chakauya, E AU - O'Keefe, BR AU - Chikwamba, Rachel K AU - Morris, L AB - The glycans on HIV-1 gp120 play an important role in shielding neutralization sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study we investigated the interaction of the lectin griffithsin (GRFT) with HIV-1 gp120 and its effects on exposure of the CD4-binding site (CD4bs). We found that GRFT enhanced the binding of HIV-1 onto plates coated with anti-CD4bs antibodies b12, b6 or the CD4 receptor mimetic, CD4-IgG2. The average enhancement of b12 or b6 binding was higher for subtype B viruses compared to subtype C while for CD4-IgG2 it was similar for both subtypes, although lower than observed with antibodies. This GRFT-mediated enhancement of HIV-1 binding to b12 was reflected in synergistic neutralization for 2 of the 4 viruses tested. The glycan at position 386 which shields the CD4bs was involved in both GRFT mediated enhancement of binding as well as neutralization synergism between GRFT and b12. Although GRFT enhanced CD4bs exposure, it simultaneously inhibited ligand binding to the coreceptor binding site, suggesting that GRFT-dependent enhancement and neutralization utilize independent mechanisms. This study shows for the first time that GRFT interaction with gp120 exposes the CD4bs through binding the glycan at position 386 which may have implications for how to access this conserved site. DA - 2011-09 DB - ResearchSpace DP - CSIR KW - Griffithsin KW - HIV-1 subtype C KW - IgG1b12 KW - CD4 binding site KW - Glycans LK - https://researchspace.csir.co.za PY - 2011 SM - 0022-538X T1 - Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site TI - Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site UR - http://hdl.handle.net/10204/6755 ER -