Mugwanda, KanganwiroHamese, SaltielVan Zyl, Winschau FPrinsloo, EDu Plessis, MDicks, LThimiri Govindaraj, Deepak B2023-03-172023-03-172023-01Mugwanda, K., Hamese, S., Van Zyl, W.F., Prinsloo, E., Du Plessis, M., Dicks, L. & Thimiri Govindaraj, D.B. 2023. Recent advances in genetic tools for engineering probiotic lactic acid bacteria. <i>Bioscience Reports, 43(1).</i> http://hdl.handle.net/10204/126781573-49350144-8463DOI: 10.1042/BSR20211299http://hdl.handle.net/10204/12678Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One of the emerging applications of synthetic biology is to exploit the link between microorganisms, biologics, and human health. To exploit this link, it is critical to select effective synthetic biology tools for use in appropriate microorganisms that would address unmet needs in human health through the development of new game-changing applications and by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered appropriate chassis organisms that can be genetically engineered for therapeutic and industrial applications. Here, we have reviewed comprehensively various synthetic biology techniques for engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, and recombineering. In addition, we also discussed heterologous protein expression systems used in engineering probiotic LAB. By combining computational biology with genetic engineering, there is a lot of potential to develop next-generation synthetic LAB with capabilities to address bottlenecks in industrial scale-up and complex biologics production. Recently, we started working on Lactochassis project where we aim to develop next generation synthetic LAB for biomedical application.FulltextenClustered regularly interspaced short palindromic repeatsCRISPRHomologous recombinationLactic acid bacteriaProbiotic engineeringSynthetic biologyArticleMugwanda, K., Hamese, S., Van Zyl, W. F., Prinsloo, E., Du Plessis, M., Dicks, L., & Thimiri Govindaraj, D. B. (2023). Recent advances in genetic tools for engineering probiotic lactic acid bacteria. <i>Bioscience Reports, 43(1)</i>, http://hdl.handle.net/10204/12678Mugwanda, Kanganwiro, Saltiel Hamese, Winschau F Van Zyl, E Prinsloo, M Du Plessis, L Dicks, and Deepak B Thimiri Govindaraj "Recent advances in genetic tools for engineering probiotic lactic acid bacteria." <i>Bioscience Reports, 43(1)</i> (2023) http://hdl.handle.net/10204/12678Mugwanda K, Hamese S, Van Zyl WF, Prinsloo E, Du Plessis M, Dicks L, et al. Recent advances in genetic tools for engineering probiotic lactic acid bacteria. Bioscience Reports, 43(1). 2023; http://hdl.handle.net/10204/12678.TY - Article AU - Mugwanda, Kanganwiro AU - Hamese, Saltiel AU - Van Zyl, Winschau F AU - Prinsloo, E AU - Du Plessis, M AU - Dicks, L AU - Thimiri Govindaraj, Deepak B AB - Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One of the emerging applications of synthetic biology is to exploit the link between microorganisms, biologics, and human health. To exploit this link, it is critical to select effective synthetic biology tools for use in appropriate microorganisms that would address unmet needs in human health through the development of new game-changing applications and by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered appropriate chassis organisms that can be genetically engineered for therapeutic and industrial applications. Here, we have reviewed comprehensively various synthetic biology techniques for engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, and recombineering. In addition, we also discussed heterologous protein expression systems used in engineering probiotic LAB. By combining computational biology with genetic engineering, there is a lot of potential to develop next-generation synthetic LAB with capabilities to address bottlenecks in industrial scale-up and complex biologics production. Recently, we started working on Lactochassis project where we aim to develop next generation synthetic LAB for biomedical application. DA - 2023-01 DB - ResearchSpace DP - CSIR J1 - Bioscience Reports, 43(1) KW - Clustered regularly interspaced short palindromic repeats KW - CRISPR KW - Homologous recombination KW - Lactic acid bacteria KW - Probiotic engineering KW - Synthetic biology LK - https://researchspace.csir.co.za PY - 2023 SM - 1573-4935 SM - 0144-8463 T1 - Recent advances in genetic tools for engineering probiotic lactic acid bacteria TI - Recent advances in genetic tools for engineering probiotic lactic acid bacteria UR - http://hdl.handle.net/10204/12678 ER -26475