Zawaira, APooran, ABarichievy, SChopera, D2012-03-282012-03-282011-09Zawaira, A, Pooran, A, Barichievy, S and Chopera, D. 2011. A discussion of molecular biology methods for protein engineering. Molecular Biotechnology, 36pp1073-60851559-0305http://www.springerlink.com/content/675581q2558p0535/http://hdl.handle.net/10204/5690Copyright: 2011 Humana Press. This is an ABSTRACT ONLY.A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.enMolecular biologyMolecular biology methodsProtein engineeringProtein expressionProtein expression methodsMutagenesisSite-directed mutagenesisInsertion mutagenesisDeletion mutagenesisPolymerase chain reactionMolecular cloningRecombinant protein expressionRecombinant plasmidArticleZawaira, A., Pooran, A., Barichievy, S., & Chopera, D. (2011). A discussion of molecular biology methods for protein engineering. http://hdl.handle.net/10204/5690Zawaira, A, A Pooran, S Barichievy, and D Chopera "A discussion of molecular biology methods for protein engineering." (2011) http://hdl.handle.net/10204/5690Zawaira A, Pooran A, Barichievy S, Chopera D. A discussion of molecular biology methods for protein engineering. 2011; http://hdl.handle.net/10204/5690.TY - Article AU - Zawaira, A AU - Pooran, A AU - Barichievy, S AU - Chopera, D AB - A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering. DA - 2011-09 DB - ResearchSpace DP - CSIR KW - Molecular biology KW - Molecular biology methods KW - Protein engineering KW - Protein expression KW - Protein expression methods KW - Mutagenesis KW - Site-directed mutagenesis KW - Insertion mutagenesis KW - Deletion mutagenesis KW - Polymerase chain reaction KW - Molecular cloning KW - Recombinant protein expression KW - Recombinant plasmid LK - https://researchspace.csir.co.za PY - 2011 SM - 1073-6085 SM - 1559-0305 T1 - A discussion of molecular biology methods for protein engineering TI - A discussion of molecular biology methods for protein engineering UR - http://hdl.handle.net/10204/5690 ER -