Lugongolo, Masixole YOmbinda-Lemboumba, SaturninManoto, Sello LMthunzi-Kufa, Patience2021-10-192021-10-192021-09Lugongolo, M.Y., Ombinda-Lemboumba, S., Manoto, S.L. & Mthunzi-Kufa, P. 2021. Examining HIV infected cells using optical trapping and Raman spectroscopy. http://hdl.handle.net/10204/12130 .0277-786X1996-756XDoi: 10.1117/12.2595766http://hdl.handle.net/10204/12130Manipulation of biological cells using optical trapping is a non-invasive approach in which individual living cells are examined without causing any damage because there is no direct mechanical contact with cells. Optical trapping uses a tightly focused laser beam emitted through a high numerical aperture microscope objective lens to hold microscopic particles. When using this technique, there is minimal chances of exposing cells to contamination and optically handled cells can still be utilised in downstream sterile experiments whenever necessary. In this study, optical trapping is used to trap HIV infected cells, which are then analysed by Raman spectroscopy. Raman spectroscopy as an analytical technique provides specific chemical/molecular details about a sample based on the fundamental vibrational modes of the chemicals. By combining these two light-based technologies, HIV infected TZM-bl cells were distinguished from the uninfected cells as they exhibited different molecular fingerprints. The acquired results both confirm and provide more detail to the findings of the previous study where transmission spectroscopy was used to differentiate between HIV infected and uninfected cells. This current study shows how the two cell populations differ according to the chemical/molecular composition and distribution. These results present valuable information that would be essential in the development of a label-free HIV point of care diagnostic device.AbstractenHuman immunodeficiency virusHIVOptical trappingRaman SpectroscopyConference PresentationLugongolo, M. Y., Ombinda-Lemboumba, S., Manoto, S. L., & Mthunzi-Kufa, P. (2021). Examining HIV infected cells using optical trapping and Raman spectroscopy. http://hdl.handle.net/10204/12130Lugongolo, Masixole Y, Saturnin Ombinda-Lemboumba, Sello L Manoto, and Patience Mthunzi-Kufa. "Examining HIV infected cells using optical trapping and Raman spectroscopy." <i>Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVII, San Diego, California, United States, 1-5 August 2021</i> (2021): http://hdl.handle.net/10204/12130Lugongolo MY, Ombinda-Lemboumba S, Manoto SL, Mthunzi-Kufa P, Examining HIV infected cells using optical trapping and Raman spectroscopy; 2021. http://hdl.handle.net/10204/12130 .TY - Conference Presentation AU - Lugongolo, Masixole Y AU - Ombinda-Lemboumba, Saturnin AU - Manoto, Sello L AU - Mthunzi-Kufa, Patience AB - Manipulation of biological cells using optical trapping is a non-invasive approach in which individual living cells are examined without causing any damage because there is no direct mechanical contact with cells. Optical trapping uses a tightly focused laser beam emitted through a high numerical aperture microscope objective lens to hold microscopic particles. When using this technique, there is minimal chances of exposing cells to contamination and optically handled cells can still be utilised in downstream sterile experiments whenever necessary. In this study, optical trapping is used to trap HIV infected cells, which are then analysed by Raman spectroscopy. Raman spectroscopy as an analytical technique provides specific chemical/molecular details about a sample based on the fundamental vibrational modes of the chemicals. By combining these two light-based technologies, HIV infected TZM-bl cells were distinguished from the uninfected cells as they exhibited different molecular fingerprints. The acquired results both confirm and provide more detail to the findings of the previous study where transmission spectroscopy was used to differentiate between HIV infected and uninfected cells. This current study shows how the two cell populations differ according to the chemical/molecular composition and distribution. These results present valuable information that would be essential in the development of a label-free HIV point of care diagnostic device. DA - 2021-09 DB - ResearchSpace DP - CSIR J1 - Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVII, San Diego, California, United States, 1-5 August 2021 KW - Human immunodeficiency virus KW - HIV KW - Optical trapping KW - Raman Spectroscopy LK - https://researchspace.csir.co.za PY - 2021 SM - 0277-786X SM - 1996-756X T1 - Examining HIV infected cells using optical trapping and Raman spectroscopy TI - Examining HIV infected cells using optical trapping and Raman spectroscopy UR - http://hdl.handle.net/10204/12130 ER -24972