Malabi, RudzaniLugongolo, Masixole YThobakgale, Setumo LManoto, Sello LMthunzi-Kufa, PatienceOmbinda-Lemboumba, Saturnin2019-08-142019-08-142019-02Ombinda-Lemboumba, S., Malabi, R., Lugongolo, M.Y., Thobakgale, S.L., Manoto, S.L. and Mthunzi-Kufa, P. 2019. Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement. Journal of Biophotonics, V12(7), pp 1-8.1864-0648https://onlinelibrary.wiley.com/doi/10.1002/jbio.201800349http://www.interscience.wiley.com/http://hdl.handle.net/10204/11081Copyright: 2019 Wiley Interscience. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website at http://www.interscience.wiley.com.Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical industry as well as the biological and medical fields. Combining transmission measurement with optical trapping has emerged as a powerful tool allowing stable sample trapping, while also facilitating transmittance data analysis. In this study, a near-infrared laser beam emitting at a wavelength of 1064 nm was used for both optical trapping and transmission measurement investigation of human immunodeficiency virus 1 (HIV-1) infected and uninfected TZM-bl cells. The measurements of the transmittance intensity of individual cells in solution were carried out using a home built optical trapping system combined with laser transmission setup using a single beam gradient trap. Transmittance spectral intensity patterns revealed significant differences between the HIV-1 infected and uninfected cells. This result suggests that the transmittance data analysis technique used in this study has the potential to differentiate between infected and uninfected TZM-bl cells without the use of labels. The results obtained in this study could pave a way into developing an HIV-1 label-free diagnostic tool with possible applications at the point of cenHIV-1 infectionLabel-free detectionMammalian cellsOptical rappingTransmission measurementArticleMalabi, R., Lugongolo, M. Y., Thobakgale, S. L., Manoto, S. L., Mthunzi-Kufa, P., & Ombinda-Lemboumba, S. (2019). Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement. http://hdl.handle.net/10204/11081Malabi, Rudzani, Masixole Y Lugongolo, Setumo L Thobakgale, Sello L Manoto, Patience Mthunzi-Kufa, and Saturnin Ombinda-Lemboumba "Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement." (2019) http://hdl.handle.net/10204/11081Malabi R, Lugongolo MY, Thobakgale SL, Manoto SL, Mthunzi-Kufa P, Ombinda-Lemboumba S. Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement. 2019; http://hdl.handle.net/10204/11081.TY - Article AU - Malabi, Rudzani AU - Lugongolo, Masixole Y AU - Thobakgale, Setumo L AU - Manoto, Sello L AU - Mthunzi-Kufa, Patience AU - Ombinda-Lemboumba, Saturnin AB - Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical industry as well as the biological and medical fields. Combining transmission measurement with optical trapping has emerged as a powerful tool allowing stable sample trapping, while also facilitating transmittance data analysis. In this study, a near-infrared laser beam emitting at a wavelength of 1064 nm was used for both optical trapping and transmission measurement investigation of human immunodeficiency virus 1 (HIV-1) infected and uninfected TZM-bl cells. The measurements of the transmittance intensity of individual cells in solution were carried out using a home built optical trapping system combined with laser transmission setup using a single beam gradient trap. Transmittance spectral intensity patterns revealed significant differences between the HIV-1 infected and uninfected cells. This result suggests that the transmittance data analysis technique used in this study has the potential to differentiate between infected and uninfected TZM-bl cells without the use of labels. The results obtained in this study could pave a way into developing an HIV-1 label-free diagnostic tool with possible applications at the point of c DA - 2019-02 DB - ResearchSpace DP - CSIR KW - HIV-1 infection KW - Label-free detection KW - Mammalian cells KW - Optical rapping KW - Transmission measurement LK - https://researchspace.csir.co.za PY - 2019 SM - 1864-0648 T1 - Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement TI - Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement UR - http://hdl.handle.net/10204/11081 ER -