The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines

Smith, TanyaO’Kennedy, Martha MRoss, CSLewis, NSAbolnik, C2023-03-152023-03-152023-02Smith, T., O’Kennedy, Martha M, Ross, C., Lewis, N. & Abolnik, C. 2023. The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. Frontiers in Plant Science, 14. http://hdl.handle.net/10204/126691664-462Xhttps://doi.org/10.3389/fpls.2023.1130910http://hdl.handle.net/10204/12669Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses.FulltextenNewcastle diseaseVirus-like particlesVLPAvian diseaseVaccinesThe production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccinesArticleSmith, T., O’Kennedy, Martha M, Ross, C., Lewis, N., & Abolnik, C. (2023). The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. Frontiers in Plant Science, 14, http://hdl.handle.net/10204/12669Smith, Tanya, O’Kennedy, Martha M, CS Ross, NS Lewis, and C Abolnik "The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines." Frontiers in Plant Science, 14 (2023) http://hdl.handle.net/10204/12669Smith T, O’Kennedy, Martha M, Ross C, Lewis N, Abolnik C. The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. Frontiers in Plant Science, 14. 2023; http://hdl.handle.net/10204/12669.TY - Article AU - Smith, Tanya AU - O’Kennedy, Martha M AU - Ross, CS AU - Lewis, NS AU - Abolnik, C AB - Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses. DA - 2023-02 DB - ResearchSpace DP - CSIR J1 - Frontiers in Plant Science, 14 KW - Newcastle disease KW - Virus-like particles KW - VLP KW - Avian disease KW - Vaccines LK - https://researchspace.csir.co.za PY - 2023 SM - 1664-462X T1 - The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines TI - The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines UR - http://hdl.handle.net/10204/12669 ER -26564