Naidoo, JerolenBrackin, RKhutlang, RethabileSavulescu, AMhlanga, Musa2015-10-152015-10-152014-06Naidoo, J, Brackin, R, Khutlang, R, Savulescu, A, and Mhlanga, MM. 2014. Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV. Applied RNAi: from Fundamental Research to Therapeutic Applications. : Caister Academic Press: United Kingdom978 190823 0676http://hdl.handle.net/10204/8185Copyright: Caister Academic Press:United Kindom. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website.The discovery of mammalian microRNAs (miRNAs) has greatly enhanced our appreciation for the complexity associated with the regulation of the mammalian transcriptional landscape. Endogenous miRNA pathways mediate the targeted and subtle variations in gene expression required to drive complex biological processes that must be coordinated both spatially and temporally within cells and tissues. It is not surprising then that the dysregulation of miRNA function has been implicated in various models of disease and pathogenesis. Increasing interest in miRNA function has facilitated the transfer of many existing technologies to miRNA-based formats. Expression-based tools like RNAseq and qPCR microarray technologies, as well as the use of synthetic molecules to inhibit or enhance miRNA functions have been employed to identify and characterize distinct miRNA expression profiles in various models of infection. In this chapter we take a closer look at the application of some of the existing tools for miRNA-based analyses with a focus on host–pathogen interactions. Aspects pertinent to high-content miRNA-based screens are also discussed using an HIV screening workflow as a backdrop to address the important considerations associated with miRNA-based studies.enMammalian microRNAsGene expressionHIV screeningRNAi researchBiomedical engineeringBiomaterialsDrug discoveryBook ChapterNaidoo, J., Brackin, R., Khutlang, R., Savulescu, A., & Mhlanga, M. (2014). Systems biology tools to understand the role of host miRNAs in infection: A closer look at HIV., <i>Workflow;13670</i> Caister Academic Press. http://hdl.handle.net/10204/8185Naidoo, Jerolen, R Brackin, Rethabile Khutlang, A Savulescu, and Musa Mhlanga. "Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV" In <i>WORKFLOW;13670</i>, n.p.: Caister Academic Press. 2014. http://hdl.handle.net/10204/8185.Naidoo J, Brackin R, Khutlang R, Savulescu A, Mhlanga M. Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV.. Workflow;13670. [place unknown]: Caister Academic Press; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/8185.TY - Book Chapter AU - Naidoo, Jerolen AU - Brackin, R AU - Khutlang, Rethabile AU - Savulescu, A AU - Mhlanga, Musa AB - The discovery of mammalian microRNAs (miRNAs) has greatly enhanced our appreciation for the complexity associated with the regulation of the mammalian transcriptional landscape. Endogenous miRNA pathways mediate the targeted and subtle variations in gene expression required to drive complex biological processes that must be coordinated both spatially and temporally within cells and tissues. It is not surprising then that the dysregulation of miRNA function has been implicated in various models of disease and pathogenesis. Increasing interest in miRNA function has facilitated the transfer of many existing technologies to miRNA-based formats. Expression-based tools like RNAseq and qPCR microarray technologies, as well as the use of synthetic molecules to inhibit or enhance miRNA functions have been employed to identify and characterize distinct miRNA expression profiles in various models of infection. In this chapter we take a closer look at the application of some of the existing tools for miRNA-based analyses with a focus on host–pathogen interactions. Aspects pertinent to high-content miRNA-based screens are also discussed using an HIV screening workflow as a backdrop to address the important considerations associated with miRNA-based studies. DA - 2014-06 DB - ResearchSpace DP - CSIR KW - Mammalian microRNAs KW - Gene expression KW - HIV screening KW - RNAi research KW - Biomedical engineering KW - Biomaterials KW - Drug discovery LK - https://researchspace.csir.co.za PY - 2014 SM - 978 190823 0676 T1 - Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV TI - Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV UR - http://hdl.handle.net/10204/8185 ER -