Theron, JCilliers, Jacques EDu Preez, MBrozel, VSVenter, SN2007-03-142007-06-072007-03-142007-06-072000-09Theron, J, et al. 2000. Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure. Journal of Applied Microbiology, vol. 89(3), pp 539-5461364-5072http://hdl.handle.net/10204/1892http://hdl.handle.net/10204/1892A pit-stop semi-nested PCR assay for the detection of toxigenic Vibrio cholerae in environmental water samples was developed and its performance evaluated. The PCR technique amplifies sequences within the cholera toxin operon specific for toxigenic V. cholerae. The PCR procedure coupled with an enrichment culture detected as few as four V. cholerae organisms in pure culture. Treated sewage, surface, ground and drinking water samples were seeded with V, cholerae and following enrichment, a detection limit of as few as 1 V. cholerae cfu ml(-1) was obtained with amplification reactions from crude bacterial lysates. The proposed method, which includes a combination of enrichment, rapid sample preparation and a pit-stop semi-nested PCR, could be applicable in the rapid detection of toxigenic V, cholerae in environmental water samples1741504 bytesapplication/pdfenCopyright: 2000 Blackwell Science LtdPCR proceduresWater samplesVibrio choleraeBroth cultivationArticleTheron, J., Cilliers, J. E., Du Preez, M., Brozel, V., & Venter, S. (2000). Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure. http://hdl.handle.net/10204/1892Theron, J, Jacques E Cilliers, M Du Preez, VS Brozel, and SN Venter "Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure." (2000) http://hdl.handle.net/10204/1892Theron J, Cilliers JE, Du Preez M, Brozel V, Venter S. Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure. 2000; http://hdl.handle.net/10204/1892.TY - Article AU - Theron, J AU - Cilliers, Jacques E AU - Du Preez, M AU - Brozel, VS AU - Venter, SN AB - A pit-stop semi-nested PCR assay for the detection of toxigenic Vibrio cholerae in environmental water samples was developed and its performance evaluated. The PCR technique amplifies sequences within the cholera toxin operon specific for toxigenic V. cholerae. The PCR procedure coupled with an enrichment culture detected as few as four V. cholerae organisms in pure culture. Treated sewage, surface, ground and drinking water samples were seeded with V, cholerae and following enrichment, a detection limit of as few as 1 V. cholerae cfu ml(-1) was obtained with amplification reactions from crude bacterial lysates. The proposed method, which includes a combination of enrichment, rapid sample preparation and a pit-stop semi-nested PCR, could be applicable in the rapid detection of toxigenic V, cholerae in environmental water samples DA - 2000-09 DB - ResearchSpace DP - CSIR KW - PCR procedures KW - Water samples KW - Vibrio cholerae KW - Broth cultivation LK - https://researchspace.csir.co.za PY - 2000 SM - 1364-5072 T1 - Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure TI - Detection of toxigenic vibrio cholera from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure UR - http://hdl.handle.net/10204/1892 ER -