Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool

Malabi, RudzaniManoto, Sello LOmbinda-Lemboumba, SaturninMaaza, MalikMthunzi-Kufa, Patience2019-08-152019-08-152019-02Malabi, R., Manoto, S.L., Ombinda-Lemboumba, S., Maaza, M., and Mthunzi-Kufa, P. 2019. Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool. SPIE BiOS, 2019, Moscone Centre, San Francisco, California, United States, 2-7 February 2019.https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10894/108940P/Detection-of-biological-analytes-using-surface-plasmon-resonance-as-a/10.1117/12.2509850.full?SSO=1https://doi.org/10.1117/12.2509850http://hdl.handle.net/10204/11091Copyright: 2019 SPIE. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's websiteSurface Plasmon Resonance (SPR) is a very powerful optical sensing technique that detects bimolecular binding interactions and it has turned out to be a suitable platform for clinical analysis. In biological and chemical sensing applications, SPR is used to monitor molecular binding real-time and it also promotes epitope mapping for determining biomolecular structures such as the interactions of proteins, DNA and viruses. This sensing technique also provides sensitive, label free and real-time monitoring of reactions. In this study we have built, characterized and optimized the SPR system for biosensing applications. Spectroscopy and scanning electron microscopy were used to characterize the surface of the SPR biosensor chip functionalized with antibodies. The home-built SPR system was successful in detecting biological analytes thereby paving a way into designing a label-free point-of-care (POC) diagnostic tool.enSurface Plasmon ResonanceMetal thin filmSpectroscopyScanning Electron MicroscopeBiosensorsDetection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic toolConference PresentationMalabi, R., Manoto, S. L., Ombinda-Lemboumba, S., Maaza, M., & Mthunzi-Kufa, P. (2019). Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool. SPIE. http://hdl.handle.net/10204/11091Malabi, Rudzani, Sello L Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, and Patience Mthunzi-Kufa. "Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool." (2019): http://hdl.handle.net/10204/11091Malabi R, Manoto SL, Ombinda-Lemboumba S, Maaza M, Mthunzi-Kufa P, Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool; SPIE; 2019. http://hdl.handle.net/10204/11091 .TY - Conference Presentation AU - Malabi, Rudzani AU - Manoto, Sello L AU - Ombinda-Lemboumba, Saturnin AU - Maaza, Malik AU - Mthunzi-Kufa, Patience AB - Surface Plasmon Resonance (SPR) is a very powerful optical sensing technique that detects bimolecular binding interactions and it has turned out to be a suitable platform for clinical analysis. In biological and chemical sensing applications, SPR is used to monitor molecular binding real-time and it also promotes epitope mapping for determining biomolecular structures such as the interactions of proteins, DNA and viruses. This sensing technique also provides sensitive, label free and real-time monitoring of reactions. In this study we have built, characterized and optimized the SPR system for biosensing applications. Spectroscopy and scanning electron microscopy were used to characterize the surface of the SPR biosensor chip functionalized with antibodies. The home-built SPR system was successful in detecting biological analytes thereby paving a way into designing a label-free point-of-care (POC) diagnostic tool. DA - 2019-02 DB - ResearchSpace DP - CSIR KW - Surface Plasmon Resonance KW - Metal thin film KW - Spectroscopy KW - Scanning Electron Microscope KW - Biosensors LK - https://researchspace.csir.co.za PY - 2019 T1 - Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool TI - Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool UR - http://hdl.handle.net/10204/11091 ER -