Griffith, Derek JBone, Emma LThomalla, Sandy JBernard, Stewart2019-04-022019-04-022018-07Griffith, D.J. et al. 2018. Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. Optics Express, vol. 26(15): 18863-188771094-4087https://www.osapublishing.org/oe/abstract.cfm?uri=oe-26-15-18863&origin=searchhttps://doi.org/10.1364/OE.26.018863http://hdl.handle.net/10204/10899This is an open access article published in Optics Express, vol. 26(15): 18863-18877A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample.enMulti-excitation fluorometerMFLPhytoplankton speciesArticleGriffith, D. J., Bone, E. L., Thomalla, S. J., & Bernard, S. (2018). Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. http://hdl.handle.net/10204/10899Griffith, Derek J, Emma L Bone, Sandy J Thomalla, and Stewart Bernard "Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield." (2018) http://hdl.handle.net/10204/10899Griffith DJ, Bone EL, Thomalla SJ, Bernard S. Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. 2018; http://hdl.handle.net/10204/10899.TY - Article AU - Griffith, Derek J AU - Bone, Emma L AU - Thomalla, Sandy J AU - Bernard, Stewart AB - A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample. DA - 2018-07 DB - ResearchSpace DP - CSIR KW - Multi-excitation fluorometer KW - MFL KW - Phytoplankton species LK - https://researchspace.csir.co.za PY - 2018 SM - 1094-4087 T1 - Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield TI - Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield UR - http://hdl.handle.net/10204/10899 ER -