Brady, DJordaan, J2010-04-082010-04-082009-07-10Brady, D and Jordaan, J 2009. Advances in enzyme immobilisation. Biotechnology Letters, Vol. 31(11), pp 1639-16500141-5492http://www.springerlink.com/content/g71665208l264352/fulltext.htmlhttp://www.springerlink.com/content/g71665208l264352/http://hdl.handle.net/10204/4008Copyright: 2009 Springer Netherlands. This is the author’s post print version. It is posted here by permission of Springer Netherlands for your personal use. Not for redistribution. The definitive version has been published in the Journal of Biotechnology Letters, Vol. 31(11), pp 1639-1650Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.enEnzymeBiocatalysisBiocatalystImmobilisation enzymeSepabeadsImmobilisationHetero-functionalised supportsEncapsulationEntrapmentSupport based immobilisationSelf immobilisationArticleBrady, D., & Jordaan, J. (2009). Advances in enzyme immobilisation. http://hdl.handle.net/10204/4008Brady, D, and J Jordaan "Advances in enzyme immobilisation." (2009) http://hdl.handle.net/10204/4008Brady D, Jordaan J. Advances in enzyme immobilisation. 2009; http://hdl.handle.net/10204/4008.TY - Article AU - Brady, D AU - Jordaan, J AB - Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications. DA - 2009-07-10 DB - ResearchSpace DP - CSIR KW - Enzyme KW - Biocatalysis KW - Biocatalyst KW - Immobilisation enzyme KW - Sepabeads KW - Immobilisation KW - Hetero-functionalised supports KW - Encapsulation KW - Entrapment KW - Support based immobilisation KW - Self immobilisation LK - https://researchspace.csir.co.za PY - 2009 SM - 0141-5492 T1 - Advances in enzyme immobilisation TI - Advances in enzyme immobilisation UR - http://hdl.handle.net/10204/4008 ER -