Steenkamp, Lucia HBrady, D2007-02-062007-06-072007-02-062007-06-072003-03-03Steenkamp, L.H. and Brady, D. Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. Enzyme and Microbial Technology, vol. 32, 04 March, pp 472-4770141-0229http://hdl.handle.net/10204/1495http://hdl.handle.net/10204/1495This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the conversion will reduce the mass of material that requires racemisation and recycling. Hence the aim was to find an enzyme that yields (S)-naproxen with an enantiomeric excess of more than 98%, a substrate conversion in excess of 40% of the race mate, and an E of >100. (R, S)-Naproxen ethyl ester (NEE) (50 mg) was used as substrate for enzyme hydrolysis reactions at 37 degrees for 4 h. Biocatalyst screening was performed in buffered aqueous solvent on a I ml scale. The reactions were stopped with 2 ml MeCN, filtered through cotton wool and analysed by HPLC to determine the percentage m/m and R/S ratio. Eight commercially available enzymes were selected for optimisation of enantioselectivity through statistically designed experiments where the reaction conditions were varied. ChiroCLEC-CR from Altus and ESL001-01 from Diversa provided acceptable enantiomeric excess, but only ChiroCLEC-CR met the specification set for the enantiomeric ratio (E).84125 bytesapplication/pdfenCopyright: 2003 Elsevier Science IncEnantioselectivityEnzyme hydrolyses reactionsNaproxenEnzymesEsteraseLipaseArticleSteenkamp, L. H., & Brady, D. (2003). Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. http://hdl.handle.net/10204/1495Steenkamp, Lucia H, and D Brady "Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester." (2003) http://hdl.handle.net/10204/1495Steenkamp LH, Brady D. Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. 2003; http://hdl.handle.net/10204/1495.TY - Article AU - Steenkamp, Lucia H AU - Brady, D AB - This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the conversion will reduce the mass of material that requires racemisation and recycling. Hence the aim was to find an enzyme that yields (S)-naproxen with an enantiomeric excess of more than 98%, a substrate conversion in excess of 40% of the race mate, and an E of >100. (R, S)-Naproxen ethyl ester (NEE) (50 mg) was used as substrate for enzyme hydrolysis reactions at 37 degrees for 4 h. Biocatalyst screening was performed in buffered aqueous solvent on a I ml scale. The reactions were stopped with 2 ml MeCN, filtered through cotton wool and analysed by HPLC to determine the percentage m/m and R/S ratio. Eight commercially available enzymes were selected for optimisation of enantioselectivity through statistically designed experiments where the reaction conditions were varied. ChiroCLEC-CR from Altus and ESL001-01 from Diversa provided acceptable enantiomeric excess, but only ChiroCLEC-CR met the specification set for the enantiomeric ratio (E). DA - 2003-03-03 DB - ResearchSpace DP - CSIR KW - Enantioselectivity KW - Enzyme hydrolyses reactions KW - Naproxen KW - Enzymes KW - Esterase KW - Lipase LK - https://researchspace.csir.co.za PY - 2003 SM - 0141-0229 T1 - Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester TI - Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester UR - http://hdl.handle.net/10204/1495 ER -