Chauke, Sipho HHlekelele, LeratoMaphanga, CharlesTjale, MabotseDube, FSOmbinda-Lemboumba, SaturninMthunzi-Kufa, P2026-04-152026-04-1520262699-9293https://doi.org/10.1002/adpr.202500229http://hdl.handle.net/10204/14782Current diagnostic tools for multidrug-resistant tuberculosis (MDR-TB) are molecular assay-based and have challenges associated with labor-intensive workflows, complex laboratory infrastructures, and limited mutation coverage. This highlights the need for alternative techniques that can be used as diagnostic tools for MDR-TB. In this study, we demonstrated the use of an surface plasmon resonance (SPR)-based biosensor chip for the detection of selected genes (InhA, KatG, and RpoB) within the MDR-TB genome using single-stranded deoxyribonucleic acids (ssDNA) targets and thiolated probes. The probes were successfully functionalized to AuNPs and confirmed using UV–vis and DLS. On SPR-based detection, the hybridization of the selected probes to complementary and non-complementary targets induced changes in the resonance angles. The hybridization of the selected probes to the targets was observed at resonance angles of 46.85, 46.77, 45.84, and 46.91° for the IS6110, InhA, KatG, and RpoB genes, respectively. In contrast, the unhybridized probe and the non-complementary targets exhibited resonance angles of 46.33, 46.05, 45.53, and 45.85° for the IS6110, InhA, KatG, and RpoB genes, respectively. The data showed that SPR-based biosensing can be refined and considered as an alternative approach to detect and differentiate between different ssDNA targets using thiolated probes as biorecognition elements for MDR-TB detection.FulltextenMultidrug-resistant tuberculosisMDR-TBSurface plasmon resonanceSPRArticleN/A