Chauke, Sipho HTjale, Mabotse AMaphanga, Charles PDube, FOmbinda-Lemboumba, SaturninMthunzi-Kufa, P2026-01-142026-01-142025-07978-1-0492-1907-3http://hdl.handle.net/10204/14587Early detection and treatment of tuberculosis (TB) remain key strategies to reduce transmission and disease progression. However, this is hampered by time-consuming, insensitive diagnostic methods, particularly for the detection of drug-resistant forms and in patients with human immunodeficiency virus infection (HIV). Several genes, such as the RpoB and InhA genes, contain mutations that are responsible for drug resistance. This study aimed to use an SPR-based biosensor platform to detect RpoB and InhA genes. DNA probes, specific to RpoB and InhA, were used as biorecognition elements to capture the corresponding target DNA sequences. The RpoB and InhA gene-specific thiolated DNA probes were immobilized on a gold-coated glass substrate before the target DNA was introduced for detection. As a negative control, a non-specific target to both genes was used to confirm the binding of the specific target. The shifts in the resonance angles indicated the binding properties associated with DNA hybridization between the specific target and the capture probe. The results obtained from this study demonstrated the use of a simple SPR setup and its potential for identifying genes associated with drug-resistant TB.FulltextenTuberculosisTBMulti-drug-resistant TBTB drugsConference Presentationn/a