Genthe, BettinaGericke, MBateman, BMjoli, NKfir, R2007-03-262007-06-072007-03-262007-06-071995Genthe, B, et al. 1995. Detection of enteric Adenoviruses in South-African waters using gene probes. Water Science and Technology, vol. 31, 06 May, pp 345-3500273-1223http://hdl.handle.net/10204/2047http://hdl.handle.net/10204/2047Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.296994 bytesapplication/pdfenCopyright: 1995 Pergamon-Elsevier Science LtdGene probesEnteric adenovirusesSephadex columnsCellulose fibreGeneclean(TM)Removal of inhibitory substancesEnvironmental sciencesWater resourcesArticleGenthe, B., Gericke, M., Bateman, B., Mjoli, N., & Kfir, R. (1995). Detection of enteric Adenoviruses in South-African waters using gene probes. http://hdl.handle.net/10204/2047Genthe, Bettina, M Gericke, B Bateman, N Mjoli, and R Kfir "Detection of enteric Adenoviruses in South-African waters using gene probes." (1995) http://hdl.handle.net/10204/2047Genthe B, Gericke M, Bateman B, Mjoli N, Kfir R. Detection of enteric Adenoviruses in South-African waters using gene probes. 1995; http://hdl.handle.net/10204/2047.TY - Article AU - Genthe, Bettina AU - Gericke, M AU - Bateman, B AU - Mjoli, N AU - Kfir, R AB - Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles. DA - 1995 DB - ResearchSpace DP - CSIR KW - Gene probes KW - Enteric adenoviruses KW - Sephadex columns KW - Cellulose fibre KW - Geneclean(TM) KW - Removal of inhibitory substances KW - Environmental sciences KW - Water resources LK - https://researchspace.csir.co.za PY - 1995 SM - 0273-1223 T1 - Detection of enteric Adenoviruses in South-African waters using gene probes TI - Detection of enteric Adenoviruses in South-African waters using gene probes UR - http://hdl.handle.net/10204/2047 ER -