Chhiba, Varsha PMathiba, KgamaVan der Westhuyzen, Christiaan WSteenkamp, Paul ARashamuse, JKStoychev, Stoyan HBode, MLBrady, D2019-04-022019-04-022018-12Chhiba, V.P. et al. 2018. Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous. Applied Microbiology and Biotechnology, vol. 102(23): doi: 10.1007/s00253-018-9367-90175-75981432-0614https://link.springer.com/article/10.1007%2Fs00253-018-9367-9https://rdcu.be/bsinBhttp://hdl.handle.net/10204/10898Copyright: 2018 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://link.springer.com/article/10.1007%2Fs00253-018-9367-9. A free fulltext non-print version of the article can be viewed at https://rdcu.be/bsinBNitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase inducer, dimethylformamide (DMF), during cultivation of two Rhodococcus rhodochrous strains (ATCC BAA-870 and PPPPB BD-1780), as well as a closely related organism (Pimelobacter simplex PPPPB BD-1781). Benzonitrile, a known nitrilase inducer, was ineffective in these strains. Biocatalytic product profiling, enzyme inhibition studies and protein sequencing were performed to distinguish the nitrilase activity from that of sequential nitrile hydratase-amidase activity. The expressed enzyme, a 40-kDa protein with high sequence similarity to nitrilase protein Uniprot Q-03217, hydrolyzed 3-cyanopyridine to produce nicotinic acid exclusively in strains BD-1780 and BD-1781. These strains were capable of synthesising both the vitamin nicotinic acid as well as ß-amino acids, a compound class of pharmaceutical interest. The induced nitrilase demonstrated high enantioselectivity (> 99%) in the hydrolysis of 3-amino-3-phenylpropanenitrile to the corresponding carboxylic acid.enBiocatalysisDimethylformamideExpressionInductionNitrilasePimelobacter simplexRhodococcus rhodochrousArticleChhiba, V. P., Mathiba, K., Van der Westhuyzen, C. W., Steenkamp, P. A., Rashamuse, J., Stoychev, S. H., ... Brady, D. (2018). Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous. http://hdl.handle.net/10204/10898Chhiba, Varsha P, Kgama Mathiba, Christiaan W Van der Westhuyzen, Paul A Steenkamp, JK Rashamuse, Stoyan H Stoychev, ML Bode, and D Brady "Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous." (2018) http://hdl.handle.net/10204/10898Chhiba VP, Mathiba K, Van der Westhuyzen CW, Steenkamp PA, Rashamuse J, Stoychev SH, et al. Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous. 2018; http://hdl.handle.net/10204/10898.TY - Article AU - Chhiba, Varsha P AU - Mathiba, Kgama AU - Van der Westhuyzen, Christiaan W AU - Steenkamp, Paul A AU - Rashamuse, JK AU - Stoychev, Stoyan H AU - Bode, ML AU - Brady, D AB - Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase inducer, dimethylformamide (DMF), during cultivation of two Rhodococcus rhodochrous strains (ATCC BAA-870 and PPPPB BD-1780), as well as a closely related organism (Pimelobacter simplex PPPPB BD-1781). Benzonitrile, a known nitrilase inducer, was ineffective in these strains. Biocatalytic product profiling, enzyme inhibition studies and protein sequencing were performed to distinguish the nitrilase activity from that of sequential nitrile hydratase-amidase activity. The expressed enzyme, a 40-kDa protein with high sequence similarity to nitrilase protein Uniprot Q-03217, hydrolyzed 3-cyanopyridine to produce nicotinic acid exclusively in strains BD-1780 and BD-1781. These strains were capable of synthesising both the vitamin nicotinic acid as well as ß-amino acids, a compound class of pharmaceutical interest. The induced nitrilase demonstrated high enantioselectivity (> 99%) in the hydrolysis of 3-amino-3-phenylpropanenitrile to the corresponding carboxylic acid. DA - 2018-12 DB - ResearchSpace DP - CSIR KW - Biocatalysis KW - Dimethylformamide KW - Expression KW - Induction KW - Nitrilase KW - Pimelobacter simplex KW - Rhodococcus rhodochrous LK - https://researchspace.csir.co.za PY - 2018 SM - 0175-7598 SM - 1432-0614 T1 - Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous TI - Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous UR - http://hdl.handle.net/10204/10898 ER -