Mokoka, TAPeter, Xolani KZimmermann, SHata, YAdams, MKaiser, MMoodley, NMaharaj, VKoorbanally, Nicoli AHamburger, MBrun, BFouché, Gerda2013-09-302013-09-302013-08Mokoka, T.A, Peter, X.K, Zimmermann, S, Hata, Y, Adams, M, Kaiser, M, Moodley, N, Maharaj, V, Koorbanally, N.A, Hamburger, M, Brun, R and Fouche, G. 2013. Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II. Planta Medica, vol. 70, pp 1-50032-0943https://www.thieme-connect.com/DOI/DOI?10.1055/s-0033-1350691http://hdl.handle.net/10204/6967Copyright: 2013 Georg Thieme Verlag. This is an ABSTRACT ONLY. The definitive version is published in Planta Medica, vol. 70, pp 1-5Two hundred and seven extracts were prepared from sixty plants from South Africa and screened for in vitro activity against Trypanosoma brucei rhodesiense, Trypanosoma cruzi, Leishmania donovani, and Plasmodium falciparum. For the 21 extracts which inhibited the growth of one or more parasites with more than 95 % at 10 µg/mL, the IC(sub)50 values against all four protozoal parasites and cytotoxic IC(sub)50 values against L6 myoblasts were determined. Amongst the most notable results are the activities of Psoralea pinnata (IC(sub)50 of 0.15 µg/mL), Schkuhria pinnata (2.04 µg/mL), and Vernonia mespilifolia (1.01 µg/mL) against Trypansoma brucei rhodesiense. HPLC-based activity profiling was used to identify the active constituents in the extracts, and the germacranolide sesquiterpene lactones schkuhrin I and II from S. pinnata, and cynaropicrin from V. mespilifolia were identified, with IC(sub)50 values of 0.9, 1.5, and 0.23 µM, respectively.enPlant extract libraryScreeningAntiprotozoal activitySesquiterpene lactonesTrypanosoma cruziLeishmania donovaniPlasmodium falciparumPsoralea pinnata (Fabaceae)Schkuhria pinnata (Asteraceae)Vernonia mespilifolia (Asteraceae)ArticleMokoka, T., Peter, X. K., Zimmermann, S., Hata, Y., Adams, M., Kaiser, M., ... Fouché, G. (2013). Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II. http://hdl.handle.net/10204/6967Mokoka, TA, Xolani K Peter, S Zimmermann, Y Hata, M Adams, M Kaiser, N Moodley, et al "Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II." (2013) http://hdl.handle.net/10204/6967Mokoka T, Peter XK, Zimmermann S, Hata Y, Adams M, Kaiser M, et al. Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II. 2013; http://hdl.handle.net/10204/6967.TY - Article AU - Mokoka, TA AU - Peter, Xolani K AU - Zimmermann, S AU - Hata, Y AU - Adams, M AU - Kaiser, M AU - Moodley, N AU - Maharaj, V AU - Koorbanally, Nicoli A AU - Hamburger, M AU - Brun, B AU - Fouché, Gerda AB - Two hundred and seven extracts were prepared from sixty plants from South Africa and screened for in vitro activity against Trypanosoma brucei rhodesiense, Trypanosoma cruzi, Leishmania donovani, and Plasmodium falciparum. For the 21 extracts which inhibited the growth of one or more parasites with more than 95 % at 10 µg/mL, the IC(sub)50 values against all four protozoal parasites and cytotoxic IC(sub)50 values against L6 myoblasts were determined. Amongst the most notable results are the activities of Psoralea pinnata (IC(sub)50 of 0.15 µg/mL), Schkuhria pinnata (2.04 µg/mL), and Vernonia mespilifolia (1.01 µg/mL) against Trypansoma brucei rhodesiense. HPLC-based activity profiling was used to identify the active constituents in the extracts, and the germacranolide sesquiterpene lactones schkuhrin I and II from S. pinnata, and cynaropicrin from V. mespilifolia were identified, with IC(sub)50 values of 0.9, 1.5, and 0.23 µM, respectively. DA - 2013-08 DB - ResearchSpace DP - CSIR KW - Plant extract library KW - Screening KW - Antiprotozoal activity KW - Sesquiterpene lactones KW - Trypanosoma cruzi KW - Leishmania donovani KW - Plasmodium falciparum KW - Psoralea pinnata (Fabaceae) KW - Schkuhria pinnata (Asteraceae) KW - Vernonia mespilifolia (Asteraceae) LK - https://researchspace.csir.co.za PY - 2013 SM - 0032-0943 T1 - Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II TI - Antiprotozoal Screening of 60 South African plants, and the identification of the Antitrypanosomal Germacranolides Schkuhrin I and II UR - http://hdl.handle.net/10204/6967 ER -