Fok, Ezio TScholefield, JanineFanucchi, StephanieMhlanga, Musa2018-05-112018-05-112017-10Fok, E.T. et al. 2017. The emerging molecular biology toolbox for the study of long noncoding RNA biology. Epigenomics, vol. 9(10): 1317-13271750-1911DOI: 10.2217/epi-2017-0062https://www.futuremedicine.com/doi/abs/10.2217/epi-2017-0062http://hdl.handle.net/10204/10194Copyright: 2017 Musa M MhlangaLong noncoding RNAs (lncRNAs) have been implicated in many biological processes. However, due to the unique nature of lncRNAs and the consequential difficulties associated with their characterization, there is a growing disparity between the rate at which lncRNAs are being discovered and the assignment of biological function to these transcripts. Here we present a molecular biology toolbox equipped to help dissect aspects of lncRNA biology and reveal functionality. We outline an approach that begins with a broad survey of genome-wide, high-throughput datasets to identify potential lncRNA candidates and then narrow the focus on specific methods that are well suited to interrogate the transcripts of interest more closely. This involves the use of imaging-based strategies to validate these candidates and observe the behaviors of these transcripts at single molecule resolution in individual cells. We also describe the use of gene editing tools and interactome capture techniques to interrogate functionality and infer mechanism, respectively. With the emergence of lncRNAs as important molecules in healthy and diseased cellular function, it remains crucial to deepen our understanding of their biology.enCRISPR/Cas9Epigenetic regulationFunctional genomicsGene transcriptionLong noncoding RNASingle molecule FISHArticleFok, E. T., Scholefield, J., Fanucchi, S., & Mhlanga, M. (2017). The emerging molecular biology toolbox for the study of long noncoding RNA biology. http://hdl.handle.net/10204/10194Fok, Ezio T, Janine Scholefield, Stephanie Fanucchi, and Musa Mhlanga "The emerging molecular biology toolbox for the study of long noncoding RNA biology." (2017) http://hdl.handle.net/10204/10194Fok ET, Scholefield J, Fanucchi S, Mhlanga M. The emerging molecular biology toolbox for the study of long noncoding RNA biology. 2017; http://hdl.handle.net/10204/10194.TY - Article AU - Fok, Ezio T AU - Scholefield, Janine AU - Fanucchi, Stephanie AU - Mhlanga, Musa AB - Long noncoding RNAs (lncRNAs) have been implicated in many biological processes. However, due to the unique nature of lncRNAs and the consequential difficulties associated with their characterization, there is a growing disparity between the rate at which lncRNAs are being discovered and the assignment of biological function to these transcripts. Here we present a molecular biology toolbox equipped to help dissect aspects of lncRNA biology and reveal functionality. We outline an approach that begins with a broad survey of genome-wide, high-throughput datasets to identify potential lncRNA candidates and then narrow the focus on specific methods that are well suited to interrogate the transcripts of interest more closely. This involves the use of imaging-based strategies to validate these candidates and observe the behaviors of these transcripts at single molecule resolution in individual cells. We also describe the use of gene editing tools and interactome capture techniques to interrogate functionality and infer mechanism, respectively. With the emergence of lncRNAs as important molecules in healthy and diseased cellular function, it remains crucial to deepen our understanding of their biology. DA - 2017-10 DB - ResearchSpace DP - CSIR KW - CRISPR/Cas9 KW - Epigenetic regulation KW - Functional genomics KW - Gene transcription KW - Long noncoding RNA KW - Single molecule FISH LK - https://researchspace.csir.co.za PY - 2017 SM - 1750-1911 T1 - The emerging molecular biology toolbox for the study of long noncoding RNA biology TI - The emerging molecular biology toolbox for the study of long noncoding RNA biology UR - http://hdl.handle.net/10204/10194 ER -