Kfir, RDu Preez, MGenthe, Bettina2007-04-022007-06-072007-04-022007-06-071993Kfir, R, Du Preez, M and Genthe, B. 1993. Use of monoclonal-antibodies for the detection of fecal bacteria in water. Water Science and Technology, vol. 27, 04 March, pp 257-2600273-1223http://hdl.handle.net/10204/2180http://hdl.handle.net/10204/2180Monoclonal antibodies (MAbs) against heat-killed Escherichia coli and Klebsiella oxytoca originating from wastewater effluent were raised in BALB/C mice. The fusion was highly successful and three hybridomas cloned were selected to study the affinity and specificity pattern of the MAbs. The MAbs were found to react equally well with heat-killed and live bacteria when tested against their original immunogens. The MAbs showed lower specificity towards environmentally isolated faecal bacteria than a pure bacterial strain grown in the laboratory. A commercially obtained MAb showed the same specificity pattern.254813 bytesapplication/pdfenCopyright: 1993 Pergamon-Elsevier LtdMonoclonal antibodiesEscherichia coliKlebsiellaFecal coliformArticleKfir, R., Du Preez, M., & Genthe, B. (1993). Use of monoclonal-antibodies for the detection of fecal bacteria in water. http://hdl.handle.net/10204/2180Kfir, R, M Du Preez, and Bettina Genthe "Use of monoclonal-antibodies for the detection of fecal bacteria in water." (1993) http://hdl.handle.net/10204/2180Kfir R, Du Preez M, Genthe B. Use of monoclonal-antibodies for the detection of fecal bacteria in water. 1993; http://hdl.handle.net/10204/2180.TY - Article AU - Kfir, R AU - Du Preez, M AU - Genthe, Bettina AB - Monoclonal antibodies (MAbs) against heat-killed Escherichia coli and Klebsiella oxytoca originating from wastewater effluent were raised in BALB/C mice. The fusion was highly successful and three hybridomas cloned were selected to study the affinity and specificity pattern of the MAbs. The MAbs were found to react equally well with heat-killed and live bacteria when tested against their original immunogens. The MAbs showed lower specificity towards environmentally isolated faecal bacteria than a pure bacterial strain grown in the laboratory. A commercially obtained MAb showed the same specificity pattern. DA - 1993 DB - ResearchSpace DP - CSIR KW - Monoclonal antibodies KW - Escherichia coli KW - Klebsiella KW - Fecal coliform LK - https://researchspace.csir.co.za PY - 1993 SM - 0273-1223 T1 - Use of monoclonal-antibodies for the detection of fecal bacteria in water TI - Use of monoclonal-antibodies for the detection of fecal bacteria in water UR - http://hdl.handle.net/10204/2180 ER -