Manoto, Sello LMalabi, RudzaniOmbinda-Lemboumba, SaturninMthunzi-Kufa, Patience2019-10-282019-10-282019-03Manoto, S.L. et al. 2019. Biocongugation of gold nanoparticles for surface plasmon resonance sensor. In: Proceedings of SPIE 10894, Plasmonics in Biology and Medicine XVI, San Francisco, California, USA, March 2019978151062431397815106243061605-74222410-9045https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10894/108941D/Biocongugation-of-gold-nanoparticles-for-surface-plasmon-resonance-sensor/10.1117/12.2509952.fullhttps://doi.org/10.1117/12.2509952http://hdl.handle.net/10204/11193Copyright: 2019 SPIE. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website.Over the past few years gold nanoparticle (AuNPs) have become extremely interesting because they possess enhanced optical, electrical and chemical properties. AuNPs have the ability to form robust conjugates with biomolecules such as antibodies and can enhance optical signals making them suitable for a variety of diagnostic applications including the surface plasmon resonance (SPR) technique. SPR is a highly sensitive and label free optical technique which is widely used for detecting biological analytes and analysing the interaction between different types of biomolecules. In this study, bioconjugation was achieved by covalently attaching antibodies to AuNPs and gold coated slides were used as SPR sensor chips in Kretschmann configuration. Several UV/VIS excitation spectra were collected before and after AuNPs were conjugated to antibodies. The results showed that the sensitivity of the SPR system significantly increased because of the bioconjugation of antibodies to AuNPs and this is a promising approach for biosensing applications.enBioconjugationgold nanoparticlessurface plasmon resonanceConference PresentationManoto, S. L., Malabi, R., Ombinda-Lemboumba, S., & Mthunzi-Kufa, P. (2019). Biocongugation of gold nanoparticles for surface plasmon resonance sensor. SPIE. http://hdl.handle.net/10204/11193Manoto, Sello L, Rudzani Malabi, Saturnin Ombinda-Lemboumba, and Patience Mthunzi-Kufa. "Biocongugation of gold nanoparticles for surface plasmon resonance sensor." (2019): http://hdl.handle.net/10204/11193Manoto SL, Malabi R, Ombinda-Lemboumba S, Mthunzi-Kufa P, Biocongugation of gold nanoparticles for surface plasmon resonance sensor; SPIE; 2019. http://hdl.handle.net/10204/11193 .TY - Conference Presentation AU - Manoto, Sello L AU - Malabi, Rudzani AU - Ombinda-Lemboumba, Saturnin AU - Mthunzi-Kufa, Patience AB - Over the past few years gold nanoparticle (AuNPs) have become extremely interesting because they possess enhanced optical, electrical and chemical properties. AuNPs have the ability to form robust conjugates with biomolecules such as antibodies and can enhance optical signals making them suitable for a variety of diagnostic applications including the surface plasmon resonance (SPR) technique. SPR is a highly sensitive and label free optical technique which is widely used for detecting biological analytes and analysing the interaction between different types of biomolecules. In this study, bioconjugation was achieved by covalently attaching antibodies to AuNPs and gold coated slides were used as SPR sensor chips in Kretschmann configuration. Several UV/VIS excitation spectra were collected before and after AuNPs were conjugated to antibodies. The results showed that the sensitivity of the SPR system significantly increased because of the bioconjugation of antibodies to AuNPs and this is a promising approach for biosensing applications. DA - 2019-03 DB - ResearchSpace DP - CSIR KW - Bioconjugation KW - gold nanoparticles KW - surface plasmon resonance LK - https://researchspace.csir.co.za PY - 2019 SM - 9781510624313 SM - 9781510624306 SM - 1605-7422 SM - 2410-9045 T1 - Biocongugation of gold nanoparticles for surface plasmon resonance sensor TI - Biocongugation of gold nanoparticles for surface plasmon resonance sensor UR - http://hdl.handle.net/10204/11193 ER -