Gbashi, SNjobeh, PSteenkamp, PTutu, HMadala, N2017-05-122017-05-122016-06Gbashi, S., Njobeh, P., Steenkamp, P. Tutu, H. Madala N. The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa. Chemistry Central JournaL, Vol.10 (37), pp. DOI 10.1186/s13065-016-0182-z1752-153Xhttp://hdl.handle.net/10204/9008https://ccj.springeropen.com/articles/10.1186/s13065-016-0182-zCopyright: 2016 The Authors.Pressurized hot water extraction (PHWE) technique has recently gain much attention for the extraction of biologically active compounds from plant tissues for analytical purposes, due to the limited use of organic solvents, its cost-effectiveness, ease-of-use and efficiency. An increase in temperature results in higher yields, however, issues with degradation of some metabolites (e.g. tartrate esters) when PHWE is conditioned at elevated temperatures has greatly limited its use. In this study, we considered possibilities of optimizing PHWE of some specific functional metabolites from Bidens pilosa using solvent compositions of 0, 20, 40 and 60 % methanol and a temperature profile of 50, 100 and 150 °C.enPressurized hot water extractionCo-solventBidens pilosaDicaffeoylquinic acidChicoric acidResponse surface modelingArticleGbashi, S., Njobeh, P., Steenkamp, P., Tutu, H., & Madala, N. (2016). The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa. http://hdl.handle.net/10204/9008Gbashi, S, P Njobeh, P Steenkamp, H Tutu, and N Madala "The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa." (2016) http://hdl.handle.net/10204/9008Gbashi S, Njobeh P, Steenkamp P, Tutu H, Madala N. The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa. 2016; http://hdl.handle.net/10204/9008.TY - Article AU - Gbashi, S AU - Njobeh, P AU - Steenkamp, P AU - Tutu, H AU - Madala, N AB - Pressurized hot water extraction (PHWE) technique has recently gain much attention for the extraction of biologically active compounds from plant tissues for analytical purposes, due to the limited use of organic solvents, its cost-effectiveness, ease-of-use and efficiency. An increase in temperature results in higher yields, however, issues with degradation of some metabolites (e.g. tartrate esters) when PHWE is conditioned at elevated temperatures has greatly limited its use. In this study, we considered possibilities of optimizing PHWE of some specific functional metabolites from Bidens pilosa using solvent compositions of 0, 20, 40 and 60 % methanol and a temperature profile of 50, 100 and 150 °C. DA - 2016-06 DB - ResearchSpace DP - CSIR KW - Pressurized hot water extraction KW - Co-solvent KW - Bidens pilosa KW - Dicaffeoylquinic acid KW - Chicoric acid KW - Response surface modeling LK - https://researchspace.csir.co.za PY - 2016 SM - 1752-153X T1 - The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa TI - The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa UR - http://hdl.handle.net/10204/9008 ER -