Ranchod, HeenaNdlandla, Fortunate LLemmer, YolandyBeukes, MNiebuhr, JAl-Dulayymi, J2018-09-062018-09-062018-08Ranchod, H. et al. 2018. The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies. PLoS ONE, vol. 13(8): https://doi.org/10.1371/journal.pone.02002981932-6203https://doi.org/10.1371/journal.pone.0200298http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200298http://hdl.handle.net/10204/10398This is an open access article.Mycolic acids (MA) are major, species-specific lipid components of Mycobacteria and related genera. In Mycobacterium tuberculosis, it is made up of alpha-, methoxy- and keto-MA, each with specific biological functions and conformational characteristics. Antibodies in tuberculosis (TB) patient sera respond differently towards the three MA classes and were reported to cross-react with cholesterol. To understand the antigenicity and cholesterol cross-reactivity of MA, we generated three different chicken-derived phage-displayed single-chain variable fragments (scFv) that reacted similarly towards the natural mixture of MA, but the first recognized all three classes of chemically synthetic MAs, the second only the two oxygenated types of MAs and the third only methoxy MA. The cholesterol cross-reactivity was investigated after grafting each of the three scFv types onto two configurations of constant chain domains±CH1-4 and CH2-4. Weak but significant cross-reactivity with cholesterol was found only with CH2-4 versions, notably those two that were also able to recognize the trans-keto MA. The cholesteroid nature of mycobacterial mycolic acids therefore seems to be determined by the trans-keto MA subclass. The significantly weaker binding to cholesterol in comparison to MA confirms the potential TB diagnostic application of these antibodies.enMycolic acidsMAMycobacterium tuberculosistuberculosisTBArticleRanchod, H., Ndlandla, F. L., Lemmer, Y., Beukes, M., Niebuhr, J., & Al-Dulayymi, J. (2018). The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies. http://hdl.handle.net/10204/10398Ranchod, Heena, Fortunate L Ndlandla, Yolandy Lemmer, M Beukes, J Niebuhr, and J Al-Dulayymi "The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies." (2018) http://hdl.handle.net/10204/10398Ranchod H, Ndlandla FL, Lemmer Y, Beukes M, Niebuhr J, Al-Dulayymi J. The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies. 2018; http://hdl.handle.net/10204/10398.TY - Article AU - Ranchod, Heena AU - Ndlandla, Fortunate L AU - Lemmer, Yolandy AU - Beukes, M AU - Niebuhr, J AU - Al-Dulayymi, J AB - Mycolic acids (MA) are major, species-specific lipid components of Mycobacteria and related genera. In Mycobacterium tuberculosis, it is made up of alpha-, methoxy- and keto-MA, each with specific biological functions and conformational characteristics. Antibodies in tuberculosis (TB) patient sera respond differently towards the three MA classes and were reported to cross-react with cholesterol. To understand the antigenicity and cholesterol cross-reactivity of MA, we generated three different chicken-derived phage-displayed single-chain variable fragments (scFv) that reacted similarly towards the natural mixture of MA, but the first recognized all three classes of chemically synthetic MAs, the second only the two oxygenated types of MAs and the third only methoxy MA. The cholesterol cross-reactivity was investigated after grafting each of the three scFv types onto two configurations of constant chain domains±CH1-4 and CH2-4. Weak but significant cross-reactivity with cholesterol was found only with CH2-4 versions, notably those two that were also able to recognize the trans-keto MA. The cholesteroid nature of mycobacterial mycolic acids therefore seems to be determined by the trans-keto MA subclass. The significantly weaker binding to cholesterol in comparison to MA confirms the potential TB diagnostic application of these antibodies. DA - 2018-08 DB - ResearchSpace DP - CSIR KW - Mycolic acids KW - MA KW - Mycobacterium tuberculosis KW - tuberculosis KW - TB LK - https://researchspace.csir.co.za PY - 2018 SM - 1932-6203 T1 - The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies TI - The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies UR - http://hdl.handle.net/10204/10398 ER -