Van de Venter, MRoux, SBungu, LCLouw, JCrouch, NRGrace, OMMaharaj, VPillay, PSewnarian, PBhagwandin, NFolb, P2009-05-182009-05-182008-09Van de Venter, M, Roux, S, Bungu, LC et al. 2008. Antidiabetic screening and scoring of eleven plants traditionally used in South Africa. Journal of Ethnopharmacology, Vol. (2008), pp 270378-8741http://hdl.handle.net/10204/3381Author Posting. Copyright Elsevier, 2008. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistributionThe aim of the paper is to investigate the traditional antidiabetic uses of indigenous or naturalised South African plants using an optimised screening and scoring method. Eleven plant species were screened against Chang liver, 3T3-L1 adipose and C2C12 muscle cells measuring glucose utilisation in all three cell lines and toxicity in the hepatocytes and adipocytes only. A scoring system was devised to aid interpretation of results. Catharanthus roseus results correlated with previously reported in vivo results, with best stimulation of glucose utilisation in hepatocytes. Momordica foetida and Momordica balsamina extracts were active in myocytes but only the latter stimulated glucose utilisation in hepatocytes. Brachylaena discolor gave the best overall results, with all plant parts giving high activity scores and negligible toxicity. In vitro toxicity results for Catharanthus roseus, Vinca major, Momordica balsamina and some Sclerocarya birrea extracts raise concern for chronic use. This screening system increases the likelihood of identifying drug candidates using in vitro antidiabetic screening of crude plant extracts, whilst the scoring system aids data interpretation. Ethnopharmacological relevance: The multitude of metabolic steps affected by Type II diabetes offer many drug targets but they complicate in vitro screening to validate traditional uses or find newdrug leads from plants.enMedicinal plantsGlucose utilisationChang liverSouth Africa3T3-L1 adiposeC2C12 muscle cellsAntidiabeticPlant extractsIndigenous plantsDiabetes discovery platformArticleVan de Venter, M., Roux, S., Bungu, L., Louw, J., Crouch, N., Grace, O., ... Folb, P. (2008). Antidiabetic screening and scoring of eleven plants traditionally used in South Africa. http://hdl.handle.net/10204/3381Van de Venter, M, S Roux, LC Bungu, J Louw, NR Crouch, OM Grace, V Maharaj, et al "Antidiabetic screening and scoring of eleven plants traditionally used in South Africa." (2008) http://hdl.handle.net/10204/3381Van de Venter M, Roux S, Bungu L, Louw J, Crouch N, Grace O, et al. Antidiabetic screening and scoring of eleven plants traditionally used in South Africa. 2008; http://hdl.handle.net/10204/3381.TY - Article AU - Van de Venter, M AU - Roux, S AU - Bungu, LC AU - Louw, J AU - Crouch, NR AU - Grace, OM AU - Maharaj, V AU - Pillay, P AU - Sewnarian, P AU - Bhagwandin, N AU - Folb, P AB - The aim of the paper is to investigate the traditional antidiabetic uses of indigenous or naturalised South African plants using an optimised screening and scoring method. Eleven plant species were screened against Chang liver, 3T3-L1 adipose and C2C12 muscle cells measuring glucose utilisation in all three cell lines and toxicity in the hepatocytes and adipocytes only. A scoring system was devised to aid interpretation of results. Catharanthus roseus results correlated with previously reported in vivo results, with best stimulation of glucose utilisation in hepatocytes. Momordica foetida and Momordica balsamina extracts were active in myocytes but only the latter stimulated glucose utilisation in hepatocytes. Brachylaena discolor gave the best overall results, with all plant parts giving high activity scores and negligible toxicity. In vitro toxicity results for Catharanthus roseus, Vinca major, Momordica balsamina and some Sclerocarya birrea extracts raise concern for chronic use. This screening system increases the likelihood of identifying drug candidates using in vitro antidiabetic screening of crude plant extracts, whilst the scoring system aids data interpretation. Ethnopharmacological relevance: The multitude of metabolic steps affected by Type II diabetes offer many drug targets but they complicate in vitro screening to validate traditional uses or find newdrug leads from plants. DA - 2008-09 DB - ResearchSpace DP - CSIR KW - Medicinal plants KW - Glucose utilisation KW - Chang liver KW - South Africa KW - 3T3-L1 adipose KW - C2C12 muscle cells KW - Antidiabetic KW - Plant extracts KW - Indigenous plants KW - Diabetes discovery platform LK - https://researchspace.csir.co.za PY - 2008 SM - 0378-8741 T1 - Antidiabetic screening and scoring of eleven plants traditionally used in South Africa TI - Antidiabetic screening and scoring of eleven plants traditionally used in South Africa UR - http://hdl.handle.net/10204/3381 ER -