Crocker, HGorda, BPelosse, MThimiri Govindaraj, Deepak BBerger, IOwens, R.J2021-10-222021-10-222021-05Crocker, H., Gorda, B., Pelosse, M., Thimiri Govindaraj, D. & Berger, I. 2021. SynBac: Enhanced baculovirus genomes by iterative recombineering. In <i>Structural Proteomics</i>. R.J. Owens, Ed. S.l.: Springer, Humana. http://hdl.handle.net/10204/12136 .978-1-0716-1406-8978-1-0716-1405-1DOI https://doi.org/10.1007/978-1-0716-1406-8_7http://hdl.handle.net/10204/12136Baculovirus expression vector systems (BEVS) are widely used to produce heterologous proteins for a wide range of applications. Developed more than 30 years ago, BEVS have been constantly modified to improve product quality and ease-of-use. Plasmid reagents were tailored and engineered to facilitate introduction of heterologous genes into baculoviral genomes. At the same time, detrimental modalities such as genes encoding proteases or apoptotic factors were removed to improve protein yield. Advances in DNA synthesis and manipulation now enable the engineering of part or whole synthetic baculovirus genomes, opening up new avenues to redesign and tailor the system to specific applications. Here, we describe a simple protocol for designing and constructing baculovirus genomes comprising segments of synthetic DNA through the use of iterative Red/ET homologous recombination reactions.AbstractenAutographa californica multiple nucleopolyhedrosis virusAcMNPVBaculovirus expression vector systemGenome engineeringRed/ET homologous recombinationBook ChapterCrocker, H., Gorda, B., Pelosse, M., Thimiri Govindaraj, D., & Berger, I. (2021). SynBac: Enhanced baculovirus genomes by iterative recombineering. In R.J. Owens. (Ed.), <i>Structural Proteomics</i> Springer, Humana. http://hdl.handle.net/10204/12136Crocker, H, B Gorda, M Pelosse, Deepak Thimiri Govindaraj, and I Berger. "SynBac: Enhanced baculovirus genomes by iterative recombineering" In <i>STRUCTURAL PROTEOMICS</i>, edited by R.J Owens. n.p.: Springer, Humana. 2021. http://hdl.handle.net/10204/12136.Crocker H, Gorda B, Pelosse M, Thimiri Govindaraj D, Berger I. SynBac: Enhanced baculovirus genomes by iterative recombineering. In Owens RJ, editor.. Structural Proteomics. [place unknown]: Springer, Humana; 2021. [cited yyyy month dd]. http://hdl.handle.net/10204/12136.TY - Book Chapter AU - Crocker, H AU - Gorda, B AU - Pelosse, M AU - Thimiri Govindaraj, Deepak AU - Berger, I AB - Baculovirus expression vector systems (BEVS) are widely used to produce heterologous proteins for a wide range of applications. Developed more than 30 years ago, BEVS have been constantly modified to improve product quality and ease-of-use. Plasmid reagents were tailored and engineered to facilitate introduction of heterologous genes into baculoviral genomes. At the same time, detrimental modalities such as genes encoding proteases or apoptotic factors were removed to improve protein yield. Advances in DNA synthesis and manipulation now enable the engineering of part or whole synthetic baculovirus genomes, opening up new avenues to redesign and tailor the system to specific applications. Here, we describe a simple protocol for designing and constructing baculovirus genomes comprising segments of synthetic DNA through the use of iterative Red/ET homologous recombination reactions. DA - 2021-05 DB - ResearchSpace DP - CSIR ED - Owens, R.J J1 - Structural Proteomics KW - Autographa californica multiple nucleopolyhedrosis virus KW - AcMNPV KW - Baculovirus expression vector system KW - Genome engineering KW - Red/ET homologous recombination LK - https://researchspace.csir.co.za PY - 2021 SM - 978-1-0716-1406-8 SM - 978-1-0716-1405-1 T1 - SynBac: Enhanced baculovirus genomes by iterative recombineering TI - SynBac: Enhanced baculovirus genomes by iterative recombineering UR - http://hdl.handle.net/10204/12136 ER -25001