Mhlongo, MIPiater, LASteenkamp, Paul AMadala, NEDubery, IA2017-07-282017-07-282015-01Mhlongo, M.I., Piater, L.A., Steenkamp, P.A. et al. 2015. Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. Biotechnology Letters, vol. 37(1): 205-209. DOI: 10.1007/s10529-014-1645-80141-5492https://link.springer.com/content/pdf/10.1007/s10529-014-1645-8.pdfDOI: 10.1007/s10529-014-1645-8https://link.springer.com/article/10.1007/s10529-014-1645-8http://hdl.handle.net/10204/9390Copyright: 2014 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to sunlight. Very little, however, is known about the biological significance and origin of cis-isomers. Here we show for the first time the accumulation of cis-5-caffeoylquinic acid in cultured tobacco cells treated with different inducers of plant defence (lipopolysaccharides, flagellin peptide-22, chitosan, acibenzolar-Smethyl and isonitrosoacetophenone), without exposure to UV light and with a 2-fold (on average) increase in the concentration of the pool in comparison to non-stimulated cells. Our UHPLC–Q-TOF-MS and multivariate statistical results suggest the presence of a possible biological pathway responsible for the production of cis-CGAs in tobacco plants.enCis-5-caffeoylquinic acidChlorogenic acidsNatural productsPhytoanticipinsPlantsSecondary metabolismTobacco cellsArticleMhlongo, M., Piater, L., Steenkamp, P. A., Madala, N., & Dubery, I. (2015). Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. http://hdl.handle.net/10204/9390Mhlongo, MI, LA Piater, Paul A Steenkamp, NE Madala, and IA Dubery "Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid." (2015) http://hdl.handle.net/10204/9390Mhlongo M, Piater L, Steenkamp PA, Madala N, Dubery I. Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. 2015; http://hdl.handle.net/10204/9390.TY - Article AU - Mhlongo, MI AU - Piater, LA AU - Steenkamp, Paul A AU - Madala, NE AU - Dubery, IA AB - Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to sunlight. Very little, however, is known about the biological significance and origin of cis-isomers. Here we show for the first time the accumulation of cis-5-caffeoylquinic acid in cultured tobacco cells treated with different inducers of plant defence (lipopolysaccharides, flagellin peptide-22, chitosan, acibenzolar-Smethyl and isonitrosoacetophenone), without exposure to UV light and with a 2-fold (on average) increase in the concentration of the pool in comparison to non-stimulated cells. Our UHPLC–Q-TOF-MS and multivariate statistical results suggest the presence of a possible biological pathway responsible for the production of cis-CGAs in tobacco plants. DA - 2015-01 DB - ResearchSpace DP - CSIR KW - Cis-5-caffeoylquinic acid KW - Chlorogenic acids KW - Natural products KW - Phytoanticipins KW - Plants KW - Secondary metabolism KW - Tobacco cells LK - https://researchspace.csir.co.za PY - 2015 SM - 0141-5492 T1 - Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid TI - Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid UR - http://hdl.handle.net/10204/9390 ER -