O'Kennedy, Maretha MBurger, JTWatson, TG2007-06-292007-06-291998-04O'Kennedy, MM, Burger, JT and Watson, TG. 1998. Stable transformation of Hi-II maize using the particle inflow gun. South African Journal of Science, Vol. South African Journal of Science 94(4), pp 188-1920038-2353http://hdl.handle.net/10204/812Copyright: 1998 Bureau Scientific PublFocuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture and plant growth conditions; Optimization of bombardment conditions by transient expression; Southern blot analysis.enEmbryogenic type II callusTransgenic plantsImmature embryosBombardmentGenetic engineeringStable transformation of Hi-II maize using the particle inflow gunArticleO'Kennedy, M. M., Burger, J., & Watson, T. (1998). Stable transformation of Hi-II maize using the particle inflow gun. http://hdl.handle.net/10204/812O'Kennedy, Maretha M, JT Burger, and TG Watson "Stable transformation of Hi-II maize using the particle inflow gun." (1998) http://hdl.handle.net/10204/812O'Kennedy MM, Burger J, Watson T. Stable transformation of Hi-II maize using the particle inflow gun. 1998; http://hdl.handle.net/10204/812.TY - Article AU - O'Kennedy, Maretha M AU - Burger, JT AU - Watson, TG AB - Focuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture and plant growth conditions; Optimization of bombardment conditions by transient expression; Southern blot analysis. DA - 1998-04 DB - ResearchSpace DP - CSIR KW - Embryogenic type II callus KW - Transgenic plants KW - Immature embryos KW - Bombardment KW - Genetic engineering LK - https://researchspace.csir.co.za PY - 1998 SM - 0038-2353 T1 - Stable transformation of Hi-II maize using the particle inflow gun TI - Stable transformation of Hi-II maize using the particle inflow gun UR - http://hdl.handle.net/10204/812 ER -