Van Zyl, WHLodolo, EJGericke, M2007-06-122007-06-121993-04Van Zyl, WH, Lodolo, EJ and Gericke, M. 1993. Conversion of homothallic yeast to heterothallism trough HO gene disruption. Current Genetics, vol. 23(4), pp 290-2940172-8083http://hdl.handle.net/10204/582Copyright: 1993 Springer-VerlagA simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeaststrains to heterothallism through HO gene disruption. An integrative ho:: neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho:: neo allele yielded G418-resistantyeast transformants in which one of the HO alleles was replaced by the disrupted ho: : neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistanttransformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho:: neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis.enG418 resistanceS. cerevisiaeHomothallismHO gene disruptionsArticleVan Zyl, W., Lodolo, E., & Gericke, M. (1993). Conversion of homothallic yeast to heterothallism trough HO gene disruption. http://hdl.handle.net/10204/582Van Zyl, WH, EJ Lodolo, and M Gericke "Conversion of homothallic yeast to heterothallism trough HO gene disruption." (1993) http://hdl.handle.net/10204/582Van Zyl W, Lodolo E, Gericke M. Conversion of homothallic yeast to heterothallism trough HO gene disruption. 1993; http://hdl.handle.net/10204/582.TY - Article AU - Van Zyl, WH AU - Lodolo, EJ AU - Gericke, M AB - A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeaststrains to heterothallism through HO gene disruption. An integrative ho:: neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho:: neo allele yielded G418-resistantyeast transformants in which one of the HO alleles was replaced by the disrupted ho: : neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistanttransformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho:: neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis. DA - 1993-04 DB - ResearchSpace DP - CSIR KW - G418 resistance KW - S. cerevisiae KW - Homothallism KW - HO gene disruptions LK - https://researchspace.csir.co.za PY - 1993 SM - 0172-8083 T1 - Conversion of homothallic yeast to heterothallism trough HO gene disruption TI - Conversion of homothallic yeast to heterothallism trough HO gene disruption UR - http://hdl.handle.net/10204/582 ER -