Ramlucken, UraishaRoets, YrielleRamchuran, Santosh OMoonsamy, GhaneshreeVan Rensburg, CJThantsh, MSLalloo, Rajesh2020-10-082020-10-082020-06Ramlucken, U. et al. 2020. Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry. Journal of General and Applied Microbiology, vol. 66, no. 4, pp. 228-2380022-12601349-8037https://europepmc.org/article/med/32536637DOI: 10.2323/jgam.2019.11.002http://hdl.handle.net/10204/11615Copyright: 2020 Microbiology Research Foundation. This is a pre-print version. The definitive version of the work is published in Journal of General and Applied Microbiology, vol. 66, no. 4, pp. 228-238Bacillus based probiotics are becoming relevant as alternatives to antibiotics used in poultry production and in other animal husbandry. This study describes the isolation of 48 Bacillus spp. candidates, from chickens and chicken environments, for use as potential probiotics in poultry production. These isolates, plus a further 18, were tested in a comprehensive in vitro screening regime that was specifically designed to select the best isolates that satisfied multiple modes of action desirable for commercial poultry probiotics. This screening programme involved the evaluation of the ability of the isolates to survive and grow in the limiting conditions of the chicken gastrointestinal tract. Only 11 of the isolates fulfilled these criteria; hence, they were further evaluated for the ability to adhere to epithelial cells, produce extracellular enzymes, and to demonstrate antagonistic activity against selected pathogens of significant importance in poultry production. Of these, a total of 6 isolates were selected, due to their all-round probiotic capability. Identification by 16S RNA sequencing confirmed these isolates as B. subtilis and B. velezensis, identities which are generally regarded as safe. The Bacillus isolates reported in our study exhibit strong all-inclusive probiotic effects and can potentially be formulated as a probiotic preparation for poultry production.enBacillus subtilisBacillus velezensisBroiler productionDevelopment of probioticsIndigenous bacteriaMulti-modeProbioticsArticleRamlucken, U., Roets, Y., Ramchuran, S. O., Moonsamy, G., Van Rensburg, C., Thantsh, M., & Lalloo, R. (2020). Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry. http://hdl.handle.net/10204/11615Ramlucken, Uraisha, Yrielle Roets, Santosh O Ramchuran, Ghaneshree Moonsamy, CJ Van Rensburg, MS Thantsh, and Rajesh Lalloo "Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry." (2020) http://hdl.handle.net/10204/11615Ramlucken U, Roets Y, Ramchuran SO, Moonsamy G, Van Rensburg C, Thantsh M, et al. Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry. 2020; http://hdl.handle.net/10204/11615.TY - Article AU - Ramlucken, Uraisha AU - Roets, Yrielle AU - Ramchuran, Santosh O AU - Moonsamy, Ghaneshree AU - Van Rensburg, CJ AU - Thantsh, MS AU - Lalloo, Rajesh AB - Bacillus based probiotics are becoming relevant as alternatives to antibiotics used in poultry production and in other animal husbandry. This study describes the isolation of 48 Bacillus spp. candidates, from chickens and chicken environments, for use as potential probiotics in poultry production. These isolates, plus a further 18, were tested in a comprehensive in vitro screening regime that was specifically designed to select the best isolates that satisfied multiple modes of action desirable for commercial poultry probiotics. This screening programme involved the evaluation of the ability of the isolates to survive and grow in the limiting conditions of the chicken gastrointestinal tract. Only 11 of the isolates fulfilled these criteria; hence, they were further evaluated for the ability to adhere to epithelial cells, produce extracellular enzymes, and to demonstrate antagonistic activity against selected pathogens of significant importance in poultry production. Of these, a total of 6 isolates were selected, due to their all-round probiotic capability. Identification by 16S RNA sequencing confirmed these isolates as B. subtilis and B. velezensis, identities which are generally regarded as safe. The Bacillus isolates reported in our study exhibit strong all-inclusive probiotic effects and can potentially be formulated as a probiotic preparation for poultry production. DA - 2020-06 DB - ResearchSpace DP - CSIR KW - Bacillus subtilis KW - Bacillus velezensis KW - Broiler production KW - Development of probiotics KW - Indigenous bacteria KW - Multi-mode KW - Probiotics LK - https://researchspace.csir.co.za PY - 2020 SM - 0022-1260 SM - 1349-8037 T1 - Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry TI - Isolation, selection and evaluation of Bacillus spp. as potential multi-mode probiotics for poultry UR - http://hdl.handle.net/10204/11615 ER -