Chetty, ASVargha, VMaity, ArjunMoolman, FSRossouw, CAnandjiwala, RBoguslavsky, LMancama, Dalubuhle TFocke, WW2013-04-172013-04-172013-02Chetty, A.S, Vargha, V, Maity, A, Moolman, F.S, Rossouw, C, Anandjiwala, R, Boguslavsky, L, Mancama, D.T. and Focke, W. 2013. Development of thermoresponsive poly (propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation. Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 419, pp 37-450927-7757http://www.sciencedirect.com/science/article/pii/S0927775712008187http://hdl.handle.net/10204/6677Copyright: 2013 Elsevier. This is an ABSTRACT ONLY. The definitive version is published in Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 419, pp 37-45Growing cells on 3D scaffolds is far superior to the conventional 2D monolayer culture method. In this study, a novel 3D thermoresponsive poly(propylene-g-N-isopropylacrylamide) (PP-g-PNIPAAm) nonwoven fabric (gNWF) was developed for cell culture using oxyfluorination-assisted graft polymerisation (OAGP). New polar functional groups were detected on the oNWF, and PNIPAAm was confirmed in the gNWF by attentuated total-reflectance Fourier transform infrared (ATR-FTIR) and scanning X-ray photoelectron spectroscopy (S-XPS). Scanning electron microscopy (SEM) revealed a rough surface morphology and confinement of the PNIPAAm graft layer to the surface of the fibres in the gNWF. The OAGP method did not affect the crystalline phase of bulk PP, however, twin-melting thermal peaks were detected for the oNWF and gNWF indicating crystal defects. Contact angle studies showed that the surface of the gNWF exhibited a thermoresponsive behaviour. Hepatocyte cells attached onto gNWF disks in a bioreactor at 37 C and remained viable for 10 days in culture. Upon cooling the cell culture media to 20 C, cells were spontaneously released as 3D multi-cellular constructs without requiring destructive enzymes. The development of 3D thermoresponsive scaffolds capable of non-invasive 3D cell culture could provide a more reliable in vitro model for cells.enOxyfluorinationGraft polymerisationSurface functionalisationPoly(propylene)Poly(N-isopropylacrylamide)ArticleChetty, A., Vargha, V., Maity, A., Moolman, F., Rossouw, C., Anandjiwala, R., ... Focke, W. (2013). Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation. http://hdl.handle.net/10204/6677Chetty, AS, V Vargha, Arjun Maity, FS Moolman, C Rossouw, R Anandjiwala, L Boguslavsky, Dalubuhle T Mancama, and WW Focke "Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation." (2013) http://hdl.handle.net/10204/6677Chetty A, Vargha V, Maity A, Moolman F, Rossouw C, Anandjiwala R, et al. Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation. 2013; http://hdl.handle.net/10204/6677.TY - Article AU - Chetty, AS AU - Vargha, V AU - Maity, Arjun AU - Moolman, FS AU - Rossouw, C AU - Anandjiwala, R AU - Boguslavsky, L AU - Mancama, Dalubuhle T AU - Focke, WW AB - Growing cells on 3D scaffolds is far superior to the conventional 2D monolayer culture method. In this study, a novel 3D thermoresponsive poly(propylene-g-N-isopropylacrylamide) (PP-g-PNIPAAm) nonwoven fabric (gNWF) was developed for cell culture using oxyfluorination-assisted graft polymerisation (OAGP). New polar functional groups were detected on the oNWF, and PNIPAAm was confirmed in the gNWF by attentuated total-reflectance Fourier transform infrared (ATR-FTIR) and scanning X-ray photoelectron spectroscopy (S-XPS). Scanning electron microscopy (SEM) revealed a rough surface morphology and confinement of the PNIPAAm graft layer to the surface of the fibres in the gNWF. The OAGP method did not affect the crystalline phase of bulk PP, however, twin-melting thermal peaks were detected for the oNWF and gNWF indicating crystal defects. Contact angle studies showed that the surface of the gNWF exhibited a thermoresponsive behaviour. Hepatocyte cells attached onto gNWF disks in a bioreactor at 37 C and remained viable for 10 days in culture. Upon cooling the cell culture media to 20 C, cells were spontaneously released as 3D multi-cellular constructs without requiring destructive enzymes. The development of 3D thermoresponsive scaffolds capable of non-invasive 3D cell culture could provide a more reliable in vitro model for cells. DA - 2013-02 DB - ResearchSpace DP - CSIR KW - Oxyfluorination KW - Graft polymerisation KW - Surface functionalisation KW - Poly(propylene) KW - Poly(N-isopropylacrylamide) LK - https://researchspace.csir.co.za PY - 2013 SM - 0927-7757 T1 - Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation TI - Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation UR - http://hdl.handle.net/10204/6677 ER -