Chhiba, Varsha PVan der Westhuyzen, Christiaan WBode, MBrady, D2019-11-252019-11-252019-06Chhiba, V.P. et al. 2019. Bacterial nitrilases and their regulation. Applied Microbiology and Biotechnology, vol. 103(12): 4679-46920175-75981432-0614https://doi.org/10.1007/s00253-019-09776-1https://link.springer.com/article/10.1007%2Fs00253-019-09776-1https://rdcu.be/bXzGihttp://hdl.handle.net/10204/11226Copyright: 2019 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://doi.org/10.1007/s00253-019-09776-1 A free fulltext non-print version of the article can be viewed at https://rdcu.be/bXzGiCommercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important components of metabolic pathways and a response to environmental factors such as natural or manmade nitriles. Nitrilases are often grouped together on a genome in specific gene clusters that reflect these metabolic functions. Although nitrilase induction systems are still poorly understood, it is known that a powerful Rhodococcal transcription regulator system permits accumulation of intracellular nitrilase of up to 30–40% of total soluble protein in wild type Rhodococcous rhodochrous and host Streptomyces strains. Nitrilase expression inducer molecules encompass a broad range of aliphatic, aromatic and heteroaromatic nitriles, as well as some secondary and tertiary amides that are resistant to nitrilase degradation.enBiocatalysisNitrilasInducerExpressionLactamsArticleChhiba, V. P., Van der Westhuyzen, C. W., Bode, M., & Brady, D. (2019). Bacterial nitrilases and their regulation. http://hdl.handle.net/10204/11226Chhiba, Varsha P, Christiaan W Van der Westhuyzen, M Bode, and D Brady "Bacterial nitrilases and their regulation." (2019) http://hdl.handle.net/10204/11226Chhiba VP, Van der Westhuyzen CW, Bode M, Brady D. Bacterial nitrilases and their regulation. 2019; http://hdl.handle.net/10204/11226.TY - Article AU - Chhiba, Varsha P AU - Van der Westhuyzen, Christiaan W AU - Bode, M AU - Brady, D AB - Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important components of metabolic pathways and a response to environmental factors such as natural or manmade nitriles. Nitrilases are often grouped together on a genome in specific gene clusters that reflect these metabolic functions. Although nitrilase induction systems are still poorly understood, it is known that a powerful Rhodococcal transcription regulator system permits accumulation of intracellular nitrilase of up to 30–40% of total soluble protein in wild type Rhodococcous rhodochrous and host Streptomyces strains. Nitrilase expression inducer molecules encompass a broad range of aliphatic, aromatic and heteroaromatic nitriles, as well as some secondary and tertiary amides that are resistant to nitrilase degradation. DA - 2019-06 DB - ResearchSpace DP - CSIR KW - Biocatalysis KW - Nitrilas KW - Inducer KW - Expression KW - Lactams LK - https://researchspace.csir.co.za PY - 2019 SM - 0175-7598 SM - 1432-0614 T1 - Bacterial nitrilases and their regulation TI - Bacterial nitrilases and their regulation UR - http://hdl.handle.net/10204/11226 ER -