Alufasi, RParawira, WStefanakis, AILebea, PChakauya, EreckChingwaru, W2020-11-062020-11-062020-08Alufasi, R. (et.al.). 2020. Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands. Environmental Technology (2020): DOI: 10.1080/09593330.2020.18113950959-33301479-487Xhttps://doi.org/10.1080/09593330.2020.1811395https://www.tandfonline.com/doi/full/10.1080/09593330.2020.1811395http://hdl.handle.net/10204/11661Copyright: 2020 Taylor & Francis. This is the abstract version of the work. For access to the fulltext, kindly visit the publisher's website.Freshwater contamination by enteric pathogens is implicated in the high frequency of diarrhoeal diseases in low to middle income countries, typically due to poor wastewater management. Constructed Wetlands are a cost-effective and sustainable alternative to conventional/mechanical treatment technologies, but the pathogen removal mechanisms in Constructed Wetlands are not fully understood. This study investigated for the first time the internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in hydroponic microcosms. Presence of Salmonella spp. within roots, rhizomes and shoots was assayed using agar-based methods over a period of 12 days. Concentration of Salmonella spp. in growth media showed 2.7 and 4.8 log unit reduction with T. latifolia and C. papyrus, respectively, and 1.8 and 6.0 log unit in unplanted units. Salmonella spp. was recovered from root and rhizome tissues of T. latifolia (up to 4.4 logCFU/g) and C. papyrus (up to 3.4 logCFU/g), and the bacteria were highly concentrated in the epidermis and cortex. However, Salmonella spp. was not detected in the stems and leaves of the two plant species. The present study demonstrates for the first time that these macrophytes internalise cells of Salmonella spp., which could be one pathogen removal mechanism employed by wetland plants.enCyperus papyrusInternalisationPathogen removal mechanismSalmonella sppTypha latifoliaArticleAlufasi, R., Parawira, W., Stefanakis, A., Lebea, P., Chakauya, E., & Chingwaru, W. (2020). Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands. http://hdl.handle.net/10204/11661Alufasi, R, W Parawira, AI Stefanakis, P Lebea, Ereck Chakauya, and W Chingwaru "Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands." (2020) http://hdl.handle.net/10204/11661Alufasi R, Parawira W, Stefanakis A, Lebea P, Chakauya E, Chingwaru W. Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands. 2020; http://hdl.handle.net/10204/11661.TY - Article AU - Alufasi, R AU - Parawira, W AU - Stefanakis, AI AU - Lebea, P AU - Chakauya, Ereck AU - Chingwaru, W AB - Freshwater contamination by enteric pathogens is implicated in the high frequency of diarrhoeal diseases in low to middle income countries, typically due to poor wastewater management. Constructed Wetlands are a cost-effective and sustainable alternative to conventional/mechanical treatment technologies, but the pathogen removal mechanisms in Constructed Wetlands are not fully understood. This study investigated for the first time the internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in hydroponic microcosms. Presence of Salmonella spp. within roots, rhizomes and shoots was assayed using agar-based methods over a period of 12 days. Concentration of Salmonella spp. in growth media showed 2.7 and 4.8 log unit reduction with T. latifolia and C. papyrus, respectively, and 1.8 and 6.0 log unit in unplanted units. Salmonella spp. was recovered from root and rhizome tissues of T. latifolia (up to 4.4 logCFU/g) and C. papyrus (up to 3.4 logCFU/g), and the bacteria were highly concentrated in the epidermis and cortex. However, Salmonella spp. was not detected in the stems and leaves of the two plant species. The present study demonstrates for the first time that these macrophytes internalise cells of Salmonella spp., which could be one pathogen removal mechanism employed by wetland plants. DA - 2020-08 DB - ResearchSpace DP - CSIR KW - Cyperus papyrus KW - Internalisation KW - Pathogen removal mechanism KW - Salmonella spp KW - Typha latifolia LK - https://researchspace.csir.co.za PY - 2020 SM - 0959-3330 SM - 1479-487X T1 - Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands TI - Internalisation of Salmonella spp. by Typha latifolia and Cyperus papyrus in vitro and implications for pathogen removal in Constructed Wetlands UR - http://hdl.handle.net/10204/11661 ER -