Maritz, JKrieg, HMYeates, CABotes, ALBreytenbach, JC2007-02-062007-06-072007-02-062007-06-072003-10Maritz, J, et al. 2003. Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane. Biotechnology Letters, vol 25 (20), pp 1775-17810141-5492http://hdl.handle.net/10204/1516http://hdl.handle.net/10204/1516Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilized in calcium alginate beads for the enantio selective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilized biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M was utilized for bead preparation.180113 bytesapplication/pdfenCopyright: 2003 Kluwer Academic PublisherCalcium alginatesEpoxide hydrolasesCell immobilisationRhodosporidium toruloides(R,S)-1,2-epoxyoctaneArticleMaritz, J., Krieg, H., Yeates, C., Botes, A., & Breytenbach, J. (2003). Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane. http://hdl.handle.net/10204/1516Maritz, J, HM Krieg, CA Yeates, AL Botes, and JC Breytenbach "Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane." (2003) http://hdl.handle.net/10204/1516Maritz J, Krieg H, Yeates C, Botes A, Breytenbach J. Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane. 2003; http://hdl.handle.net/10204/1516.TY - Article AU - Maritz, J AU - Krieg, HM AU - Yeates, CA AU - Botes, AL AU - Breytenbach, JC AB - Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilized in calcium alginate beads for the enantio selective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilized biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M was utilized for bead preparation. DA - 2003-10 DB - ResearchSpace DP - CSIR KW - Calcium alginates KW - Epoxide hydrolases KW - Cell immobilisation KW - Rhodosporidium toruloides KW - (R,S)-1,2-epoxyoctane LK - https://researchspace.csir.co.za PY - 2003 SM - 0141-5492 T1 - Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane TI - Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane UR - http://hdl.handle.net/10204/1516 ER -