Ramchuran, Santosh OBerger, EDu Plessis, ECrampton, BGLouw, ME2009-05-052009-05-052006-09Ramchuran, SO, Berger, E, Du Plessis, E, Crampton, BG and Louw, ME. 2006. Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein. 4TH Recombinant protein production conference, Barcelona, Spain, 21–23 September 2006, pp 2http://hdl.handle.net/10204/3350This poster presentation is published by BioMed Central, and is Open AccessIn order to investigate the possibility of using the FliC display system for biotransformation in B. halodurans, construct pSECNLipC habouring the LipA gene from Geobacillus thermoleovorans was designed and evaluated in shake flasks and batch fermentations. Lipases have been well characterised and play a role in a number of processes which include detergents, glycerolysis of fats and oils, direct esterification, chiral resolution and acylate synthesis (Litthauer et al. 2002). The use of lipases immobilized on the cell surface of Staphylococcus carnosus for biotransformation has been demonstrated using the fibronectin binding protein B fused to the Staphylococcus hyicus lipaseenLipaseEnzymesBacillus halodurans Alk36FliC protein4TH Recombinant protein production conferenceProtein productionpSECNLipCB. haloduransConference PresentationRamchuran, S. O., Berger, E., Du Plessis, E., Crampton, B., & Louw, M. (2006). Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein. BioMed Central. http://hdl.handle.net/10204/3350Ramchuran, Santosh O, E Berger, E Du Plessis, BG Crampton, and ME Louw. "Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein." (2006): http://hdl.handle.net/10204/3350Ramchuran SO, Berger E, Du Plessis E, Crampton B, Louw M, Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein; BioMed Central; 2006. http://hdl.handle.net/10204/3350 .TY - Conference Presentation AU - Ramchuran, Santosh O AU - Berger, E AU - Du Plessis, E AU - Crampton, BG AU - Louw, ME AB - In order to investigate the possibility of using the FliC display system for biotransformation in B. halodurans, construct pSECNLipC habouring the LipA gene from Geobacillus thermoleovorans was designed and evaluated in shake flasks and batch fermentations. Lipases have been well characterised and play a role in a number of processes which include detergents, glycerolysis of fats and oils, direct esterification, chiral resolution and acylate synthesis (Litthauer et al. 2002). The use of lipases immobilized on the cell surface of Staphylococcus carnosus for biotransformation has been demonstrated using the fibronectin binding protein B fused to the Staphylococcus hyicus lipase DA - 2006-09 DB - ResearchSpace DP - CSIR KW - Lipase KW - Enzymes KW - Bacillus halodurans Alk36 KW - FliC protein KW - 4TH Recombinant protein production conference KW - Protein production KW - pSECNLipC KW - B. halodurans LK - https://researchspace.csir.co.za PY - 2006 T1 - Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein TI - Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein UR - http://hdl.handle.net/10204/3350 ER -