Makita, CChimuka, LCukrowska, ESteenkamp, PAKandawa-Schutz, MNdhlala, ARMadala, NE2017-05-022017-05-022017-01Makita, C., Chimuka, L., Cukrowska, E., Steenkamp, P.A., Kandawa-Schutz, M., Ndhlala, A.R. and Madala, N.E. 2017. UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts. South African Journal of Botany, Vol.108, pp. 193–199http://www.sciencedirect.com/science/article/pii/S0254629916303003http://hdl.handle.net/10204/9004Copyright: 2017 Elsevier. This is a pre-print version. The definitive version of the work is published in the South African Journal of Botany, Vol. 108 pp.193 –199Moringa ovalifolia Dinter & A. Berger (Moringaceae) is a succulent-stemmed plant, endemic to the desert and semi-desert areas of central Namibia and southwestern Angola. Just like other species in the Moringaceae family, M. ovalifolia is believed to be rich in health-promoting phytochemicals. However, there are very limited scientific reports on the phytochemical composition and associated biological activities of this plant. Chlorogenic acids (CGAs), major phenolic compounds of Moringa species, have been shown to be effective natural remedies for the management of chronic ailments such as diabetes and cardiovascular diseases. Using a UPLC-ISCID-MS/MS method optimized to mimic the MSn fragmentation of an ion trap-based MS but generating accurate mass data, various isomers of chlorogenic acids and their associated derivatives in the leaves of M. ovalifolia were profiled. M. ovalifolia was shown to contain cis and trans isomers of 3-acyl, 4-acyl and 5-acyl p-coumaroylquinic (pCoQA), caffeoylquinic (CQA) and feruloylquinic acids (FQA) (1–18), a single isomer of 3,5-diCQA (19), 3-CQA-glycoside (20) and two regional isomers of the (3' and 4') glycosides of 4-CQA (21, 22). To the best of our knowledge, this is the first report on the presence of these compounds in M.ovalifolia. The results of the current study confirmed the richness of an underutilized M. ovalifolia as a source of pharmacological relevant metabolites with potential medicinal applications.enMoringa ovalifoliaUPLC-qTOF-MSChlorogenic acidsDicaffeoylquinic acidsp-Coumaroylquinic acidsFeruloylquinic acidsCaffeoylquinic acid glycosidesUPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extractsArticleMakita, C., Chimuka, L., Cukrowska, E., Steenkamp, P., Kandawa-Schutz, M., Ndhlala, A., & Madala, N. (2017). UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts. http://hdl.handle.net/10204/9004Makita, C, L Chimuka, E Cukrowska, PA Steenkamp, M Kandawa-Schutz, AR Ndhlala, and NE Madala "UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts." (2017) http://hdl.handle.net/10204/9004Makita C, Chimuka L, Cukrowska E, Steenkamp P, Kandawa-Schutz M, Ndhlala A, et al. UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts. 2017; http://hdl.handle.net/10204/9004.TY - Article AU - Makita, C AU - Chimuka, L AU - Cukrowska, E AU - Steenkamp, PA AU - Kandawa-Schutz, M AU - Ndhlala, AR AU - Madala, NE AB - Moringa ovalifolia Dinter & A. Berger (Moringaceae) is a succulent-stemmed plant, endemic to the desert and semi-desert areas of central Namibia and southwestern Angola. Just like other species in the Moringaceae family, M. ovalifolia is believed to be rich in health-promoting phytochemicals. However, there are very limited scientific reports on the phytochemical composition and associated biological activities of this plant. Chlorogenic acids (CGAs), major phenolic compounds of Moringa species, have been shown to be effective natural remedies for the management of chronic ailments such as diabetes and cardiovascular diseases. Using a UPLC-ISCID-MS/MS method optimized to mimic the MSn fragmentation of an ion trap-based MS but generating accurate mass data, various isomers of chlorogenic acids and their associated derivatives in the leaves of M. ovalifolia were profiled. M. ovalifolia was shown to contain cis and trans isomers of 3-acyl, 4-acyl and 5-acyl p-coumaroylquinic (pCoQA), caffeoylquinic (CQA) and feruloylquinic acids (FQA) (1–18), a single isomer of 3,5-diCQA (19), 3-CQA-glycoside (20) and two regional isomers of the (3' and 4') glycosides of 4-CQA (21, 22). To the best of our knowledge, this is the first report on the presence of these compounds in M.ovalifolia. The results of the current study confirmed the richness of an underutilized M. ovalifolia as a source of pharmacological relevant metabolites with potential medicinal applications. DA - 2017-01 DB - ResearchSpace DP - CSIR KW - Moringa ovalifolia KW - UPLC-qTOF-MS KW - Chlorogenic acids KW - Dicaffeoylquinic acids KW - p-Coumaroylquinic acids KW - Feruloylquinic acids KW - Caffeoylquinic acid glycosides LK - https://researchspace.csir.co.za PY - 2017 T1 - UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts TI - UPLC-qTOF-MS profiling of pharmacologically important chlorogenic acids and associated glycosides in Moringa ovalifolia leaf extracts UR - http://hdl.handle.net/10204/9004 ER -