Henriques, RGriffiths, CRego, EHMhlanga, Musa2011-12-142011-12-142011-05Henriques, R, Griffiths, C et al. 2011. PALM and STORM: unlocking live-cell super-resolution. Biopolymers, Vol 95(5), pp 322-3310006-3525http://onlinelibrary.wiley.com/doi/10.1002/bip.21586/abstracthttp://hdl.handle.net/10204/5414Copyright: 2011 Wiley Blackwell. This is an ABSTRACT ONLYLive-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal.enSuper-resolutionMicroscopy methodsSingle moleculesPALMSTORMLive-cell imagingSuper-resolution microscopyArticleHenriques, R., Griffiths, C., Rego, E., & Mhlanga, M. (2011). PALM and STORM: unlocking live-cell super-resolution. http://hdl.handle.net/10204/5414Henriques, R, C Griffiths, EH Rego, and Musa Mhlanga "PALM and STORM: unlocking live-cell super-resolution." (2011) http://hdl.handle.net/10204/5414Henriques R, Griffiths C, Rego E, Mhlanga M. PALM and STORM: unlocking live-cell super-resolution. 2011; http://hdl.handle.net/10204/5414.TY - Article AU - Henriques, R AU - Griffiths, C AU - Rego, EH AU - Mhlanga, Musa AB - Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal. DA - 2011-05 DB - ResearchSpace DP - CSIR KW - Super-resolution KW - Microscopy methods KW - Single molecules KW - PALM KW - STORM KW - Live-cell imaging KW - Super-resolution microscopy LK - https://researchspace.csir.co.za PY - 2011 SM - 0006-3525 T1 - PALM and STORM: unlocking live-cell super-resolution TI - PALM and STORM: unlocking live-cell super-resolution UR - http://hdl.handle.net/10204/5414 ER -