Alexander, KabambaMalatji, KanyaneMulaudzi, Takalani2022-05-272022-05-272020-05Alexander, K., Malatji, K. & Mulaudzi, T. 2020. Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission. <i>Virology, 544.</i> http://hdl.handle.net/10204/124260042-6822https://doi.org/10.1016/j.virol.2020.01.017http://hdl.handle.net/10204/12426Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT against cell-free and cell-associated viruses, while 3MG and 4MG were significantly more potent. Furthermore, the restoration of the 234N or 295N glycan in these viruses, known to increase sensitivity to GRFT, also increased sensitivity to 2MG and 2MG3, and not to 3MG and 4MG. In addition, GRFT resistant viruses generated in-vitro were equally resistant to 2MG and 2MG3 while they had considerably low resistance to 3MG and 4MG. Lastly, all five compounds showed increased inhibitory activity in seminal and vaginal simulants although the effect was more pronounced in the former. These data support further studies of tandemers as potential microbicides.FulltextenLectinsHIV-1TransmissionsResistanceGriffithsinTandemersArticleAlexander, K., Malatji, K., & Mulaudzi, T. (2020). Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission. <i>Virology, 544</i>, http://hdl.handle.net/10204/12426Alexander, Kabamba, Kanyane Malatji, and Takalani Mulaudzi "Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission." <i>Virology, 544</i> (2020) http://hdl.handle.net/10204/12426Alexander K, Malatji K, Mulaudzi T. Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission. Virology, 544. 2020; http://hdl.handle.net/10204/12426.TY - Article AU - Alexander, Kabamba AU - Malatji, Kanyane AU - Mulaudzi, Takalani AB - Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT against cell-free and cell-associated viruses, while 3MG and 4MG were significantly more potent. Furthermore, the restoration of the 234N or 295N glycan in these viruses, known to increase sensitivity to GRFT, also increased sensitivity to 2MG and 2MG3, and not to 3MG and 4MG. In addition, GRFT resistant viruses generated in-vitro were equally resistant to 2MG and 2MG3 while they had considerably low resistance to 3MG and 4MG. Lastly, all five compounds showed increased inhibitory activity in seminal and vaginal simulants although the effect was more pronounced in the former. These data support further studies of tandemers as potential microbicides. DA - 2020-05 DB - ResearchSpace DP - CSIR J1 - Virology, 544 KW - Lectins KW - HIV-1 KW - Transmissions KW - Resistance KW - Griffithsin KW - Tandemers LK - https://researchspace.csir.co.za PY - 2020 SM - 0042-6822 T1 - Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission TI - Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission UR - http://hdl.handle.net/10204/12426 ER -23306