Lalloo, RajeshMaharajh, Dheepak MGörgens, JGardiner, NS2009-05-082009-05-082008-05Lalloo, R, Maharajh, D, Görgens, J and Gardiner, NS. 2008. Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture. Applied Microbiology and Biotechnology, Vol. 79(1), pp 1-260175-7598http://hdl.handle.net/10204/3363This is a post-print version of the published article, and we have been granted permission by the publisher (Springer Verlag) to archive it on the CSIR's institutional repository (Research Space)Modern aquaculture utilises intensive reticulated systems resulting in waste accumulation and proliferation of disease. Conventional chemical treatments cause resistance in pathogens and negative environmental impact. The potential of a Bacillus cereus isolate (NRRL 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. The functionality of this isolate across a range of physiological conditions, including salinity, pH and temperature, based on rearing of high-value ornamental Cyprinus carpio, was investigated. Temperature had a significant influence on germination, specific growth rate and increase in cell number of B. cereus in shake-flask cultures, while salinity and pH did not have a measurable effect on growth. Controlled studies in bioreactors and modelling of the data to the Arrhenius function indicated the existence of high and low growth temperature domains. The rates of pathogenic Aeromonas hydrophila suppression and decrease in waste ion concentrations (ammonium, nitrite, nitrate, phosphate) were translated into a linear predictive indicator of efficacy of the B. cereus isolate at different temperatures. The present study confirmed the robustness of the B. cereus isolate (NRRL 100132) as a putative biological agent for aquaculture and further demonstrated a novel method for the assessment of in vitro biological efficacy as a function of temperatureenBacillus cereusNRRL 100132AquacultureCyprinus carpioTemperature effectSalinitypHPathogensDisease controlArticleLalloo, R., Maharajh, D. M., Görgens, J., & Gardiner, N. (2008). Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture. http://hdl.handle.net/10204/3363Lalloo, Rajesh, Dheepak M Maharajh, J Görgens, and NS Gardiner "Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture." (2008) http://hdl.handle.net/10204/3363Lalloo R, Maharajh DM, Görgens J, Gardiner N. Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture. 2008; http://hdl.handle.net/10204/3363.TY - Article AU - Lalloo, Rajesh AU - Maharajh, Dheepak M AU - Görgens, J AU - Gardiner, NS AB - Modern aquaculture utilises intensive reticulated systems resulting in waste accumulation and proliferation of disease. Conventional chemical treatments cause resistance in pathogens and negative environmental impact. The potential of a Bacillus cereus isolate (NRRL 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. The functionality of this isolate across a range of physiological conditions, including salinity, pH and temperature, based on rearing of high-value ornamental Cyprinus carpio, was investigated. Temperature had a significant influence on germination, specific growth rate and increase in cell number of B. cereus in shake-flask cultures, while salinity and pH did not have a measurable effect on growth. Controlled studies in bioreactors and modelling of the data to the Arrhenius function indicated the existence of high and low growth temperature domains. The rates of pathogenic Aeromonas hydrophila suppression and decrease in waste ion concentrations (ammonium, nitrite, nitrate, phosphate) were translated into a linear predictive indicator of efficacy of the B. cereus isolate at different temperatures. The present study confirmed the robustness of the B. cereus isolate (NRRL 100132) as a putative biological agent for aquaculture and further demonstrated a novel method for the assessment of in vitro biological efficacy as a function of temperature DA - 2008-05 DB - ResearchSpace DP - CSIR KW - Bacillus cereus KW - NRRL 100132 KW - Aquaculture KW - Cyprinus carpio KW - Temperature effect KW - Salinity KW - pH KW - Pathogens KW - Disease control LK - https://researchspace.csir.co.za PY - 2008 SM - 0175-7598 T1 - Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture TI - Functionality of a bacillus cereus biological agent in response to physiological variables encountered in cyprinus carpio aquaculture UR - http://hdl.handle.net/10204/3363 ER -