Vining, KJRomanel, EJones, RCAlves-Ferreira, MHefer, CAAmarasinghe, VDharmawardhana, PNaithani, SRanik, MWesley-Smith, JSolomon, LJaiswal, PMyburg, AAStrauss, ST2015-10-302015-10-302015-10Vining, K.J, Romanel, E, Jones, R.C, Alves-Ferreira, M, Hefer, C.A, Amarasinghe, V, Dharmawardhana, P, Naithani, S, Ranik, M, Wesley-Smith, J, Solomon, L, Jaiswal, P, Myburg, A.A and Strauss, S.T. 2015. The floral transcriptome of Eucalyptus grandis. New Phytologist, vol. 208(8), pp 1406-14220028-646Xhttp://onlinelibrary.wiley.com/doi/10.1111/nph.13077/pdfhttp://hdl.handle.net/10204/8216Copyright: 2015 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in New Phytologist, vol. 208(8), pp 1406-1422As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages, and compared these with transcriptomes of diverse vegetative tissues, including leaves, roots, and stems. A subset of 4807 genes (13% of protein-coding genes) were differentially expressed between floral buds of either stage and vegetative tissues. A similar proportion of genes were differentially expressed among all tissues. A total of 479 genes were differentially expressed between early and late stages of floral development. Gene function enrichment identified 158 gene ontology classes that were overrepresented in floral tissues, including ‘pollen development’ and ‘aromatic compound biosynthetic process’. At least 40 floral-dominant genes lacked functional annotations and thus may be novel floral transcripts. We analyzed several genes and gene families in depth, including 49 putative biomarkers of floral development, the MADS-box transcription factors, ‘S-domain’-receptor-like kinases, and selected gene family members with phosphatidylethanolamine-binding protein domains. Expanded MADS-box gene subfamilies in Eucalyptus grandis included SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), SEPALLATA (SEP) and SHORT VEGETATIVE PHASE (SVP) Arabidopsis thaliana homologs. These data provide a rich resource for functional and evolutionary analysis of genes controlling eucalypt floral development, and new tools for breeding and biotechnology.enEucalyptusFloral transcriptomeMADS-boxS-domain receptorlike kinaseSDRLKArticleVining, K., Romanel, E., Jones, R., Alves-Ferreira, M., Hefer, C., Amarasinghe, V., ... Strauss, S. (2015). The floral transcriptome of Eucalyptus grandis. http://hdl.handle.net/10204/8216Vining, KJ, E Romanel, RC Jones, M Alves-Ferreira, CA Hefer, V Amarasinghe, P Dharmawardhana, et al "The floral transcriptome of Eucalyptus grandis." (2015) http://hdl.handle.net/10204/8216Vining K, Romanel E, Jones R, Alves-Ferreira M, Hefer C, Amarasinghe V, et al. The floral transcriptome of Eucalyptus grandis. 2015; http://hdl.handle.net/10204/8216.TY - Article AU - Vining, KJ AU - Romanel, E AU - Jones, RC AU - Alves-Ferreira, M AU - Hefer, CA AU - Amarasinghe, V AU - Dharmawardhana, P AU - Naithani, S AU - Ranik, M AU - Wesley-Smith, J AU - Solomon, L AU - Jaiswal, P AU - Myburg, AA AU - Strauss, ST AB - As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages, and compared these with transcriptomes of diverse vegetative tissues, including leaves, roots, and stems. A subset of 4807 genes (13% of protein-coding genes) were differentially expressed between floral buds of either stage and vegetative tissues. A similar proportion of genes were differentially expressed among all tissues. A total of 479 genes were differentially expressed between early and late stages of floral development. Gene function enrichment identified 158 gene ontology classes that were overrepresented in floral tissues, including ‘pollen development’ and ‘aromatic compound biosynthetic process’. At least 40 floral-dominant genes lacked functional annotations and thus may be novel floral transcripts. We analyzed several genes and gene families in depth, including 49 putative biomarkers of floral development, the MADS-box transcription factors, ‘S-domain’-receptor-like kinases, and selected gene family members with phosphatidylethanolamine-binding protein domains. Expanded MADS-box gene subfamilies in Eucalyptus grandis included SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), SEPALLATA (SEP) and SHORT VEGETATIVE PHASE (SVP) Arabidopsis thaliana homologs. These data provide a rich resource for functional and evolutionary analysis of genes controlling eucalypt floral development, and new tools for breeding and biotechnology. DA - 2015-10 DB - ResearchSpace DP - CSIR KW - Eucalyptus KW - Floral transcriptome KW - MADS-box KW - S-domain receptorlike kinase KW - SDRLK LK - https://researchspace.csir.co.za PY - 2015 SM - 0028-646X T1 - The floral transcriptome of Eucalyptus grandis TI - The floral transcriptome of Eucalyptus grandis UR - http://hdl.handle.net/10204/8216 ER -